参苓白术散对脾虚湿困证溃疡性结肠炎大鼠结肠菌群的影响  

The Influence of Shenlingbaizhusan on Intestinal Flora of Rats Modeled by Ulcerative Colitis of Spleen Deficiency and Dampness Type

作　　者：孙娟[1] 王键[1] 胡建鹏[1] 李姿慧[1] 叶铭钢[1] 徐伟[1] 

机构地区：[1]安徽中医药大学省部共建新安医学教育部重点实验室,安徽合肥230038

出　　处：《云南中医学院学报》2013年第4期1-4,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(NO:81173172);安徽省高等学校省级自然科学研究重点项目(NO:KJ2011 A184);安徽省"115"产业创新团队项目;安徽中医学院青年科学研究基金项目(NO:2013qn024);安徽省自然科学基金项目(NO:1308085QH155)

摘　　要：目的观察参苓白术散对脾虚湿困证溃疡性结肠炎(UC)大鼠结肠菌群的影响,探讨参苓白术散治疗UC的作用机制。方法将40只Wistar大鼠,随机分为正常对照组、脾虚湿困证UC模型组、参苓白术散组、阳性对照药组。采用传统中医学病因与现代医学病因相结合的方法复制UC模型,给药治疗后,提取结肠内容物细菌总DNA,应用肠杆菌基因间重复共有序列基因扩增(ERIC-PCR)技术进行菌群分析。结果 4组大鼠的结肠内容物菌丛存在整体差异,模型组ERIC-PCR指纹图谱DNA条带较少,存在6条分布较一致的主带;而其他3组ERIC-PCR指纹图谱DNA条带较多,且主带位置分布无统一趋势,其中参苓白术散组ERIC-PCR指纹图谱与正常组最为接近。结论 UC可能存在较单一的肠道优势菌,参苓白术散对脾虚湿困证UC结肠菌群的失衡具有明显的调整作用,可使肠道优势菌种类增加,恢复菌群平衡状态,从而促进损伤肠组织的恢复。Objective To observe the influence of Shenlingbaizhusan on intestinal flora of rats modeled by ulcerative colitis （UC）of spleen deficiency and dampness type in order to explore the therapeutic mechanism of Shenlingbaizhusan to UC. Methods 40 healthy Wistar rats were randomly divided into normal group,model group,Shenlingbaizhusan treated group,and sulfasalazine（SASP） group. The damp-heat syndrome of stomach and intestine were copied by multivariate methods which simulated the etiology of Traditional Chinese Medicine. The treatment had been given to the appropriate model. Total bacterial DNA was extracted from the contents of the colon. Flora was analyzed by ERIC-PCR technology. Results There were the differences of the intestinal flora in four groups according to the analysis. The DNA bands of ERIC-PCR fingerprinting electrophoresis were little in model group. The DNA bands of ERIC-PCR fingerprinting electrophoresis were numerous in other three groups. The trend of main-belt location of Shenlingbaizhusan was close to normal group. Conclusion There might be an advantage intestinal bacterium. Shenlingbaizhusan played an important role in adjusting microflora imbalance of UC of spleen deficiency and dampness type,Increasing dominant strain of intestinal,restoring the flora balance status in order to promote the recovery of damaged intestinal tissue.

关 键 词：参苓白术散 溃疡性结肠炎 脾虚湿困证 结肠菌群 

分 类 号：R285.5[医药卫生—中药学]