玉屏风散对上呼吸道菌群失调小鼠咽部ICAM表达的影响  

Effects of Yupingfeng San on the Expression of Mice Pharynx ICAM under Flora Imbalance in the Upper Respiratory Tract

作　　者：陈滟[1] 姚政[1] 赵文娟[1] 马洁[1] 陈文慧[1] 

机构地区：[1]云南中医学院基础医学院,云南昆明650500

出　　处：《云南中医学院学报》2013年第4期9-12,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省科技计划项目(NO:2008CD150)

摘　　要：目的研究玉屏风散对上呼吸道菌群失调致感染小鼠咽部黏膜ICAM(inter cellular adhesion molecular)表达的调节作用。方法 30只SPF级昆明种小白鼠随机分为3组:正常对照组、模型组和玉屏风散组。模型组通过腹腔注射氨苄青霉素致上呼吸道菌群失调建立模型,玉屏风散组在造模型后用玉屏风散灌胃治疗。7d后取咽部黏膜组织固定,进行ICAM免疫组化染色,显微镜下观察其表达情况,应用图像分析仪计算阳性结果的平均光密度值。结果模型组ICAM表达较对照组增加(P<0.01),而玉屏风散组ICAM表达较模型组明显减少(P<0.01)。结论菌群失调所致咽部感染会促进小鼠咽黏膜ICAM的表达,而玉屏风散能减少小鼠咽黏膜ICAM的表达。表明玉屏风散调节上呼吸道微生态失衡的作用机制可能与降低ICAM有关。Objective To study the regulation effect of Yupingfeng San on the expression of ICAM in mice pharynx mucous membrane when flora imbalance causes upper respiratory tract infection. Methods 30 SPF Kunming mice were randomly divided into three groups：normal control group, model group and Yupingfeng San group. The model group by intraperitoneal injection of ampicillin induced flora imbalance model of the upper respiratory tract. Yupingfeng San group in building models were treated by gastric perfusion With Yupingfeng San. 7 days later,the pharyngeal mucosal tissues were fixed and ICAM was measured by immunohistochemical staining in these tissues. To observe the positive expression under the microscope and calculate the average optical density of the positive results using image analyzer. Results In the model group,the expression of ICAM increased than the control group（P〈0.01）,while the expression of ICAM in Yupingfeng San group than those in the model group decreased significantly （P〈0.01）. Conclusion Pharyngeal infection by flora imbalance can promote the expression of ICAM in mouse pharyngeal mucosal tissues. But Yupingfeng San can reduce the expression of ICAM. Consequently,the adjustment mechanism of Yupingfeng San to the upper respiratory Micro-ecological imbalance may be related to the reduce of ICAM.

关 键 词：玉屏风散 上呼吸道 菌群失调 ICAM 

分 类 号：R285[医药卫生—中药学]