芸薹链格孢菌孢子萌发及附着胞形成的初步定量研究  

Initial Quantitative Studies on Spores Germination and Appressorium Formation of Alternaria brassicae

作　　者：马海霞[1,2] 刘影[2,3] 屈直[1] 李丹[2] 杨毅[2] 杨信东[2,4] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]吉林农业大学农学院,长春130118 [3]吉林省农业科学院植保所,公主岭136100 [4]吉林农业大学发展学院,长春130600

出　　处：《吉林农业大学学报》2013年第4期393-397,共5页Journal of Jilin Agricultural University

基　　金：中国热带农业科学院热带生物技术研究所中央级公益性科研项目(ITBB120301)

摘　　要：采用常规流行学方法研究了芸薹链格孢菌的孢子萌发及附着胞形成。结果表明：一般浸入水中2 h后就有少量孢子萌发,10 h孢子萌发率可达90%以上;孢子萌发时各个细胞均可萌发形成芽管,向不同方向伸长,形成附着胞,20 h附着胞形成率可达50%以上;花粉和叶面物质对孢子萌发有促进作用;孢子抗干燥能力较弱,萌发孢子只须经4 h干燥即可完全丧失存活能力;芸薹链格孢菌孢子萌发及附着胞形成的温度范围均为10-25℃,15℃为孢子萌发及附着孢形成的最适温度,温度达40℃后孢子不再萌发。The study adopted a conventional epidemic experiment on spores germination and appressorium formation.The results showed that spores of Alternaria brassicae can germinate easily in pure water,a few spores can germinate after 2 hours of immersion,and the spores of Alternaria brassicae can germinate over 90% after 10 hours.Each cell may germinate and form the tube,and elongate to different directions.The rate of appressorium can be over 50% after 20 hours.Substance in the leaf surface and pollen can accelerate the germination of spores.The spores don′t have strong ability to resist drying condition,the germinated spores completely died after 4 hours in drying condition.The temperature of the sporulation and appressorium formation were both about 10~25 ℃.It was at 15 ℃ that the ratio of spores germination and the rate of appressorium formation were the highest.The spores cannot sporulate over 40 ℃.

关 键 词：白菜黑斑病 芸薹链格孢 生态学特性 病害流行 

分 类 号：S436.34[农业科学—农业昆虫与害虫防治]