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作 者:辛雅芬[1] 米士伟[2] 张淑玲[1] 邓建玲[1] 高克祥[2]
机构地区:[1]上海农林职业技术学院园艺园林系,上海201600 [2]山东农业大学植物保护学院,泰安271018
出 处:《吉林农业大学学报》2013年第4期450-456,共7页Journal of Jilin Agricultural University
基 金:国家自然科学基金项目(30872024);上海农林职业技术学院科研课题资助项目(091126);山东省科技发展计划项目(2010GNC10911)
摘 要:利用光学显微镜、扫描电镜和透射电镜观察了植物内生真菌球毛壳ND35菌株在黄瓜品种"津研4号"根部的侵染定殖情况。菌丝主要从表皮细胞之间的缝隙侵入,在表皮细胞和外皮层细胞中定殖,可与植物建立共生关系。通过施用不同含量的球毛壳ND35菌肥,研究其对不同黄瓜品种生长和产量的影响。结果表明:0.2 g/株毛壳生物菌肥处理在显著增加黄瓜品种"津研4号"和"申绿06号"株高和叶面积的同时,提高了黄瓜产量,黄瓜单株产量分别比对照增加了43.2%和67.2%。Infection and colonization of endophytic fungus Chaetomium globosum ND35 on the root of "Jinyan No.4" cucumber were observed by light microscopy,scanning electron microscopy and transmission electron microscopy.Hyphae mainly infected root from gaps between epidermal cells and colonized in epidermal cells and outer cortical cells.It was capable of establishing symbiosis with plant.The effect of Chaetomium globosum ND35 on growth and yield of 4 cucumber varieties was investigated by applying different content of fungal biofertilizer.The result indicated that applying fungal biofertilizer at 0.2 g per plant was able to promote and increase significantly height growth and leaf area of "Jinyan No.4" and "Shenlü No.06" cucumber,and enhance their yield.Compared with the control plant,yield per plant cucumber increased by 43.2 % and 67.2%,respectively.
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