北京地区宋内志贺菌分子流行病学特征分析  

Molecular epidemiological characteristics of Shigella Sonnei isolates in Beijing

作　　者：刘桂荣[1] 曲梅[1] 张新[1] 吴晓娜[1] 李锡太[1] 贾蕾[1] 严寒秋[1] 梁志超[1] 王全意[1] 

机构地区：[1]北京市疾病预防控制中心,北京100013

出　　处：《中国热带医学》2013年第8期950-952,963,共4页China Tropical Medicine

基　　金："艾滋病和病毒性肝炎等重大传染病防治"国家科技重大专项(No.2012ZX10004215-003-001)

摘　　要：目的了解北京地区宋内志贺菌毒力基因分布及分子流行病学特征。方法采用实时荧光PCR方法检测毒力基因、脉冲场凝胶电泳(PFGE)分型,多位点序列分型(MLST)及多位点可变数目串联重复序列分析(MLVA)等方法对北京地区2001～2009年50株志贺菌菌株进行分子流行病学特征分析。结果 50株菌中,ipaH、set1、sen三种毒力基因的携带率分别为98%,18%和78%。选取19株流行病学和遗传背景无关的菌株,进行MLST、MLVA与PFGE分型方法的比较,MLST为1个序列型ST152 complex:adk(11)、fumC(63)、gyrB(7)、icd(1)、mdh(14)、purA(7)、recA(7);MLVA被分成15个型别,D值为0.9708;PFGE分成12个型别,D值为0.9532。MLVA初筛得到的8个可变数目串联重复(VNTR)位点用于扩大菌株分析,发现50株志贺菌被分为21个MLVA的型别,TS002和TS001为主要型别。结论北京地区近年来流行的宋内志贺菌存在毒力基因的丢失,MLVA分子型别复杂,存在多克隆来源,提示需要进行连续系统的分子流行病学监测。Objective To understand the distribution and epidemiological characteristics of Shigella Sonnei isolates in Beijing. Methods Fifty Shigella Sonnei strains isolated during 2001-2009 were etected for ipaH,setl and sen virulence gene by realtime PCR and molecular typed by pulsed-field gel electrophoresis （PFGE）,multilocus sequence subtyping（MisT）and multilocus variable-number tandem-repeat analysis（MLVA）. Results The distribution of ipaH, setl and sen virulence gene among 50 strains were 98%,18%和78%,respectively. Nineteen unrelated strains were selected for comparison of molecular typing methods of PFGE,MIST and MLVA. MIST results produced just one sequence type ST152 complex： adk（11 ）,fumC（63）,gyrB（7）,icd（ 1 ）,mdh（14） ,purA（7） and recA（7） ;MLVA produced 15 types with a D value of 0.9708,while PFGE for 12 types with a D value of 0.9532. Through screening 8VNTR loci,50 Shigella Sonnei isolates were classified into 21 MLVA types with a D value of 0.9708,while 130 Shigellaflexneria strains were classified into 63 MLVA types by PFGE,with 2 dominant types TS002 and TS001. Conclusions The loss of virulence genes,genetic polymorphism and multiple clones source occur to ShigeUa Sonnei isolates in Beijing in recent years,suggesting a continuous and systematic molecular epidemiological surveillance is neccessary.

关 键 词：宋内志贺菌 分子分型 多位点序列分型 多位点可变数目串联重复分析 

分 类 号：R378.25[医药卫生—病原生物学]