转化生长因子β对椎间盘细胞Ⅱ型胶原基因表达的调节作用  

Regulating effects of transforming growth factor beta (TGF β) on gene expression of collagen type Ⅱ in human intervertebral discs

作　　者：陈岩[1] 胡有谷[1] 吕振华[1] 

机构地区：[1]青岛大学医学院附属医院骨科,266003

出　　处：《中华外科杂志》2000年第9期703-706,I039,共5页Chinese Journal of Surgery

基　　金：山东省卫生厅资助项目! (1996 12 )

摘　　要：目的　探讨转化生长因子 β (TGF β)对椎间盘细胞Ⅱ型胶原基因表达的调节作用。　方法　应用原位杂交技术检测TGF β1对人胚椎间盘原代培养及传代培养的纤维环细胞、髓核细胞中Ⅱ型胶原mRNA的调节作用 ,其中TGF β1浓度分别为 0ng/ml、1ng/ml和l0ng/ml;采用VIDAS软件计算杂交涂片中细胞的平均光密度值 ,进行胶原mRNA相对定量。　结果　 (1)原代培养的椎间盘纤维环细胞 ,TGF β1浓度 1ng/ml组及 10ng/ml组其光密度值分别为 0ng/ml组的 74 6 %和 6 0 2 % ;原代培养的髓核细胞 ,TGF β1浓度 1ng/ml组及 10ng/ml组其光密度值分别是 0ng/ml组的 6 9 6 %和5 5 5 %。 (2 )传代培养的纤维环细胞 ,TGF β1浓度 1ng/ml组及 10ng/ml组其光密度值分别为 0ng/ml组的 15 1%和 16 6 % ;传代培养的髓核细胞 ,TGF β1浓度 1ng/ml组及 10ng/ml组其光密度值分别是 0ng/ml组的 145 %和 198%。　结论　TGF β对椎间盘细胞Ⅱ型胶原基因表达的调节作用取决于纤维环及髓核中类软骨细胞及脊索细胞的分化状态。对于原代培养分化较为完全的细胞 ,TGF β可以抑制Ⅱ型胶原基因表达 ;对于传代培养的、正在经历去分化的类软骨细胞及脊索细胞 ,TGF β可以刺激Ⅱ型胶原基因表达 ,提示TGFObjective [WT5”BZ]To assess the regualting effects of TGF β on gene expression of collagen type Ⅱ in the human intervertebral discs. [WT5”HZ]Methods [WT5”BZ]In situ hybridization was used to investigate the effect of TGF β1 on collagen mRNA in confluent primary and passaged monolayer cell cultures of annulus fibrosus (AF) as well as nucleus pulposus (NP). The mean photodensitometry of cell smears as semi quantitative analysis was evaluated by VIDAS software. [WT5”HZ]Results[WT5”BZ] In primary cultures,1 ng/ml and 10 ng/ml TGF β1 inhibited the collagen type Ⅱ mRNA levels by 74 6% and 60 2% respectively in AF; they also inhibited the mRNA levels by 69 6% and 55 5% respectively in NP.In passaged cultures in which the notochordal cells and chondrocytes were in dedifferentiation status, 1ng/ml and 10 ng/ml TGF β1 increased the collagen type Ⅱ mRNA levels by 151% and 166% respectively in AF and also increased the mRNA levels by 145% and 198% respectively in NP. [WT5”HZ]Conclusions [WT5”BZ]The regulation effect of TGF β on collagen type Ⅱ gene expression is dependent on whether the cells are fully differentiated or undergoing phenotype loss, and TGF β may play an important role in the repair process during early disc degeneration, especially in nucleus pulposus. [WT5”HZ]

关 键 词：转化生长因子Β 椎间盘退变 Ⅱ型胶原 MRNA 

分 类 号：R602[医药卫生—外科学]