高效液相色谱法测定野鸦椿药材中鞣花酸含量  

Determination of Ellagic Acid in Euscaphis Japonica(Thunb. ) by HPLC

作　　者：刘智[1] 王爱民[2] 许祖超 李勇军[2] 廖尚高[2] 王永林[2] 

机构地区：[1]贵阳医学院附属医院药剂科,贵州贵阳550004 [2]贵阳医学院药学院,贵州贵阳550004 [3]贵州益康制药有限公司,贵州贵阳550025

出　　处：《中国药业》2013年第17期16-18,共3页China Pharmaceuticals

基　　金：贵州省中药现代化科技产业研究开发专项资金项目;项目编号:黔科合中药专字[2007]5014号

摘　　要：目的建立测定野鸦椿药材中鞣花的含量高效液相色谱法。方法色谱柱为WaterssymmetryC18柱（150mm×4．6mm，5μm），流动相为甲醇-0．1％磷酸3z（36：64），流速1mL／min，柱温为40℃，检测波长为254nm。结果鞣花酸进样量在0．129～2．06Ixg范围内与峰面积呈良好线性关系（r=0．9994），平均加样回收率为100．57％，RSD为2．43％（n=6）。结论该方法线性关系、精密度和准确度良好，专属性强，可作为野鸦椿药材中鞣花酸的质量控制方法。Objective To establish a HPLC method for the determination of the ellagic acid content in Euscaphis Japonica（ThuMb. ）. Methods ＇File chromatographic separation was performed on the Waters symmetry C]s column （150 mm×4.6 mm, 5 μm） with methanol-0. 1% H3PO4 solution（36：64） as the mobile phase, the flow rate was 1 mL/min and the column temperature was 40 ℃. The detection wave length was 245 nm. Results The linearity of ellagic acid was in the range of 0. 129- 2.06 μg（ r =0. 999 4）, the average recovery rate was 100. 57%, RSD was 2.43% （n =6）. Conclusion This method has good linearity, precision and accuracy strong speci- ficity. It can be used for the quality control method of ellagic acid in Euscaphis Japonica（ThuMb. ）.

关 键 词：高效液相色谱法 野鸦椿 鞣花酸 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]