金银花枝叶抗猪蓝耳病毒有效活性部位中主要化学成分的含量测定  

Content Determination of the Main Chemical Constituents in the Effective AntiPRRSV Fraction of the Branches and Leaves of Lonicera japonica

作　　者：刘利青[1] 邓张双[1] 杨进[1] 陈剑锋[1] 邹坤[1] 邰源临[2] 胡建忠[2] 

机构地区：[1]三峡大学天然产物研究与利用湖北省重点实验室,宜昌443002 [2]水利部沙棘开发管理中心,北京100038

出　　处：《医药导报》2013年第9期1203-1206,共4页Herald of Medicine

基　　金：中华人民共和国水利部公益项目(SWCC2012003);三峡大学博士启动基金(KJ2008B014);国家自然科学基金面上项目(31070313);国家自然科学基金资助项目(21272136)

摘　　要：目的建立抗猪蓝耳病毒金银花枝叶的有效活性部位中4个主要化学成分:benzyl alcohol O-(6'-O-β-Dxylopyranosyl)-β-D-glucopyranoside(1),gentiopicrin(2),dihydrodehydrodiconiferyl alcohol 4'-O-β-D-glucoside(3),apigenin 5-O-β-D-glucopyranoside(4)的含量测定方法。方法采用高效液相色谱(HPLC)法,Acclaim C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,流动相为乙腈-水(10∶90)^(30∶70)变梯度洗脱,检测时间0~30 min,流速为1.0 mL·min-1,检测波长为203,210,254,280 nm。结果化合物1~4的平均加样回收率分别为98.96%(RSD=1.49%),98.73%(RSD=1.42%),98.93%(RSD=0.98%),99.14%(RSD=0.96%)。结论该方法简便,准确,重复性好,可用于抗猪蓝耳病毒金银花枝叶有效活性部位中4个主要化学成分的含量测定。Objective To establish a method for content determination of 4 chemical constituents, including benzyl alcohol O-（6＇-O-β-D-xylopyranosyl ） -β-D-glucopyranoside （ 1 ）, gentiopicrin （ 2 ）, dihydrodehydrodiconiferyl alcohol 4＇-O -β-D- glucoside （3） , and apigenin 5-O-β-D-glucopyranoside（4 ） , in the effective anti- porcine reproductie and respiratou syndrome virus （PRRSV） fraction of the branches and leaves of Lonicera japonica by HPLC. Methods The HPLC separation was performed on Acclaim C18 （4.6 mm×250 mm,5 μm） column at 30 ℃. The mobile phase in the gradient elution was acetonitrile-water （10：90-30 ： 70）, at the flow rate of 1.0 mL ·min-1 and the detection wavelength of 203,210,254 ,and 280 nm, respectively. Results The average recovery of the four ingredients was 98.96% （ RSD = 1.49% ）, 98.73% （ RSD = 1.42% ）, 98.93 % （ RSD = 0.98% ）, and 99. 14% （ RSD = 0.96% ） , respectively. Conclusion The method is simple, accurate and reproducible ,which can be used in the content determination of the four chemical constituents in the effective anti-PRRSV fraction of the branches and leaves of Lonicera japonica.

关 键 词：金银花 抗猪蓝耳病毒 化学成分 含量测定 

分 类 号：R282.71[医药卫生—中药学] R284[医药卫生—中医学]