外源植物激素对甘蓝显性雄性不育材料离体快繁的影响  

Effects of Exogenous Plant Hormones on in vitro Rapid Propagation of Dominant Genic Male Sterile Cabbages

作　　者：严慧玲[1,2] 方智远[1] 刘玉梅[1] 杨丽梅[1] 庄木[1] 张扬勇[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]河北省农林科学院经济作物研究所,河北石家庄050051

出　　处：《热带农业科学》2013年第8期29-33,共5页Chinese Journal of Tropical Agriculture

基　　金：国家十一五科技支撑计划(No.2009Bad8B00)

摘　　要：以甘蓝纯合显性不育材料(DGMS)03-01Z608-1、04Z521-12、04Z-01Z126-1为试材,研究不同激素种类和浓度以及基因型对甘蓝纯合DGMS材料快速繁殖的影响。结果表明:04Z521-12、03Z-01Z608-1最适宜增殖的激素组成是:MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,而04Z-01Z126-1最适宜增殖的培养基组成是:MS+KT 1.0mg/L+6-BA 0.5 mg/L;不同甘蓝DGMS材料在相同培养条件下,由于基因型的不同增殖系数有很大差别;圆球类型甘蓝DGMS材料04Z521-12增殖系数最高,尖球类型甘蓝DGMS材料03Z-01Z608-1增殖系数最低。In this paper, using the homozygous dominant genic male sterile （DGMS） cabbage 03-01Z608-1, 04Z521.12 and 04Z-O1Z126-1 as experimental materials, the effects of different hormone types and concentrations and genotypes on in vitro rapid propagation of homozygous DGMS cabbages were studied. The results as followed： the suitable hormone combinations for rapid propagation of 04Z521-12 and 03Z-01zr08-1 was MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L, and MS＋KT1.0 mg/L＋6-BA 0.5 mg/L for 04Z-O1Z126-1; in the same conditions, different DGMS cabbages had different propagation coefficient because of different genotypes; round-head type DGMS material 04Z521-12 had the highest propagation coefficient, while tip-head type DGMS material 03Z-01Z608-1 had the lowest propagation efficiency.

关 键 词：甘蓝 显性雄性不育 繁殖系数 

分 类 号：S436.36[农业科学—农业昆虫与害虫防治]