丹路口服液质量标准研究  

Study on Quality Standards of Danlu Oral Liquid

作　　者：郭爱枝[1] 何益峰[1] 王吉慧[1] 孙培文[1] 

机构地区：[1]常德市第一人民医院,湖南常德415003

出　　处：《中医药导报》2013年第8期82-84,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立丹路口服液质量标准。方法:采用TLC法对丹路口服液中丹参、当归、川芎、红花进行定性鉴别;以HPLC法对其赤芍中的芍药苷进行含量测定。色谱柱:Symmetry C18柱(200 mm×4.6 mm,5μm);流动相:乙腈-1‰磷酸水溶液(19∶81);流速:1.0 mL.min-1;检测波长:230 nm;柱温:30℃。结果:在TLC色谱中检出丹参、当归、川芎、红花;芍药苷进样量在6.14-61.40μg mL-1范围内线性关系良好,r=0.9998;平均加样回收率101.0%,RSD%为2.5%。结论:所建立的方法简便可行,重现性好,为丹路口服液质量控制提供了方法。Objective： To establish a quality standard for Danlu Oral Liquid.Methods： Salvia mihiorrhiza Bge,Angelica sinensis（Oliv） Diels,Ligustici Chuanxiong Hort,Carthamus tinctorius L were indentified by TLC.HPLC was applied to determine the Paeoniflorin;The separation was performed on a Symmetry of C18 column（200 mm×4.6 nnn,5 μm）;The mobile phase was consisted of acetonitrile and water（1‰ phosphoric acid）（19∶81）;The detection wavelength was 230 nm;Flow rate was 1.0 mL.min-1;Column temperature was 30 ℃.Results： Salvia miltiorrhiza Bge,Angelica sinensis（Oliv） Diels,Ligusticum chuanxiong Hort,Carthamus tinctorius L could be detected by TLC.Paeoniflorin showed a good linear relationship at 6.14-61.40 μg.mL-1,r=0.9998;The average recovery of the added index was 101.0%,and RSD% was 2.5%.Conclusion： The method is simple,feasible and reproducible.The established quality standard can be used for the quality control of this medicine.

关 键 词：丹路口服液 薄层色谱法 高效液相色谱法 芍药苷 

分 类 号：R284.1[医药卫生—中药学]