贵州9个少数民族线粒体DNA Region V及Y染色体DYS287位点多态性研究  

An Analysis on the Polymorphism of Region V of Mitochondrial DNA and Y-chromosome DYS287 Sites in 9 Ethnic Groups of Guizhou

作　　者：任凌雁[1] 何燕[1] 张婷[1] 王婵娟[1] 官志忠[1] 单可人[1] 

机构地区：[1]贵阳医学院分子生物学重点实验室,贵州贵阳550004

出　　处：《中山大学学报（自然科学版）》2013年第4期121-124,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：贵州省科技计划资助项目(黔科合J[2011]2119;黔科合SY[2010]3001;黔科合SY[2012]3135号)

摘　　要：调查贵州9个世居少数民族线粒体DNA Region V以及Y染色体DYS287位点多态性情况,为贵州相关少数民族的遗传背景研究提供可参考的分子遗传学数据。采用PCR-聚丙烯酰胺凝胶(PCR-SDS PAGE)技术、PCR-琼脂糖凝胶电泳技术检测线粒体RegionV 9 bp序列的缺失、Y染色体DYS287位点多态性。结果显示在本次研究的9个少数民族中线粒体DNA的Region V发现2种多态性,即标准型和缺失性,仡佬族、畲族、壮族、仫佬族、毛南族、蒙古族、回族、羌族、满族线粒体DNA Region V 9 bp缺失频率分别为:26.6%、25%、20%、18.3%、13.3%、10%、8.3%、6.7%、5%;Y染色体DYS287多态显示:仡佬族、仫佬族YAP+携带率分别为20%、1.67%,其余民族全部显示为YAP-。研究获得贵州9个民族人群线粒体DNA Region V及Y染色体DYS287位点多态数据,为各民族群体遗传分析及起源等提供相关遗传背景资料。This study investigated the polymorphism of Region V of the mitoehondrial DNA and Y-chro- mosome DYS287 sites in 9 ethnic groups of Guizhou to provide the referable molecular genetics data with related ethnic groups of Guizhou. Polymerase chain reaction-polyacrylamide SDS gel electrophoresis and Polymerase chain reaction-agarose gel eleetrophoresis were applied to detect the polymorphism of 9bp de- letion of mitochondrial DNA noncoding region and Y-chromosome DYS287 sites. Two type of polymor- phism in mitochondrial DNA were discovered： standard form and deletion form; the frequency of 9bp de- letion of mtDNA in Gelao, She, Zhuang, Mulao, Maonan, Menggu, Hui, Qiang, Man people were： 26.6 %, 25 %, 20 %, 18.3 %, 13.3 %, 10 %, 8.3 %, 6.7 %, 5 %, respectively; two type of polymorphism in Y-chromosome DYS287 ： YAP and YAP-, the frequency of YAP ＋ in Gelao, Mao- nan were ： 20 % , 1.67 % , respectively, others were 0. The data of polymorphism of Region V of mito- chondrial DNA and Y-chromosome DYS287 sites were obtained in 9 ethnic groups of Guizhou and the ge- netic information were provided for ethnic groups.

关 键 词：贵州 少数民族 线粒体DNA DYS287 多态性 

分 类 号：Q39[生物学—遗传学]