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作 者:黄利娜[1,2] 梁萌青[1] 张海涛[1] 曲江波 王新星[1] 郑珂珂[1]
机构地区:[1]中国水产科学研究院黄海水产研究所,青岛266071 [2]上海海洋大学水产与生命学院,201306 [3]烟台开发区天源水产有限公司,264000
出 处:《渔业科学进展》2013年第4期62-70,共9页Progress in Fishery Sciences
基 金:鲆鲽类产业技术体系科研专项经费(nycytx-50-G07)资助
摘 要:以维生素A添加量为0(对照组)、10 000IU/kg(1万组)和80 000IU/kg(8万组)的3种人工配合饲料饲养大菱鲆Scophthamus maximus亲鱼8个月,通过比较各饲料组亲鱼平均相对产卵量、卵子上浮率、受精率、孵化率、卵子与仔稚鱼的大小等,研究不同添加水平的维生素A对亲鱼繁殖性能的影响。结果表明,8万组卵子上浮率和孵化率显著高于对照组(P<0.05),与1万组差异不显著(P>0.05);8万组初孵仔鱼体长2 684.1±24.91μm,显著高于对照组2 562.3±30.00μm(P<0.05),与1万组2 484.2±56.16μm差异不显著(P>0.05);卵子中n-3HUFA、n-6HUFA的含量和性腺中维生素A的含量随着饲料中维生素A添加量的增加而增加;8万组血清中SOD(超氧化物歧化酶,Superoxide dismutase)活力为91.88U/ml,显著高于对照组70.57U/ml(P<0.05)。The eight-month experiment was conducted to determine the effects of vitamin A supplementation (0, 10000, and 80000IU/kg diet) on the growth and reproductive perform- ance of broodstock Scophthamus maximus. Average relative fecundity, percentage of buoyant eggs, fertilization rate, hatching rate of eggs, size of eggs and length of fry were used to evalu- ate the performance of turbot. The results showed that the percentage of buoyant eggs and hatchability of eggs of the group fed with 80000IU/kg vitamin A were significantly higher than those of the control group(P〈0.05). The body length of fry of the group fed with 80000IU/kg vitamin A was 2 684.1+24.91μm, which was significantly higher than those of the control (2 562.3+30.00μm) (P%0.05). The content of n-3HUFA and n-6HUFA in eggs and vitamin A in gonad increased with the increase of vitamin A supplementation levels in diet. SOD activity in serum of broodstock fed with 80000IU/kg vitamin A was 91.88U/ml, which was significantly higher than those of the control (70.57U/ml)(P〈0.05). This experiment demonstrated that vitamin A supplemented in the diets could improve the reproductive performance of turbot broodstock.
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