外周血单个核细胞内性甾体激素受体与慢性乙型肝炎肝组织病理状态的相关性  

Correlation between sex steroid hormone receptors in peripheral blood mononuclear cells and histopathology of liver tissue in patients with chronic hepatitis B

作　　者：史慧慧[1] 张小楠[2] 张占卿[1] 陆伟[1] 沈芳[3] 冯艳玲[4] 袁正宏[5] 

机构地区：[1]上海市公共卫生临床中心肝炎二科,201508 [2]上海市公共卫生临床中心科研中心,201508 [3]上海市公共卫生临床中心检验科,201508 [4]上海市公共卫生临床中心病理科,201508 [5]复旦大学教育部,卫生部医学分子病毒学重点实验室

出　　处：《肝脏》2013年第8期510-516,共7页Chinese Hepatology

摘　　要：目的探讨雌激素受体α(estrogen receptorα,ERα)mRNA和雄激素受体(androgen receptor,AR)mRNA与肝组织病理状态的相关性。方法收集111例慢性乙型肝炎患者肝组织病理标本及外周血单个核细胞,肝组织病理诊断包括炎症程度分级及纤维化分期,外周血单个核细胞提取总RNA进行反转录,以获取的cDNA为模板,运用实时定量PCR方法测定外周血单个核细胞内ERαmRNA和AR mRNA含量。结果外周血单个核细胞中AR mRNA的含量在不同的肝组织炎症分级和纤维化分期存在着显著差异,ERαmRNA含量在不同的肝组织炎症程度分级中有显著性差异。通过Logistic回归分析,得出AR mRNA是影响肝组织炎症分级的主要因素,年龄和AR mRNA两个主要因素会影响肝组织纤维化分期。结论 AR表达水平是影响慢性乙型肝炎的发病和进展的重要因素。Objective To evaluate the efficacy of estrogen receptor a（ER a） mRNA and androgen receptor （AR） mRNA correlated with histopatbology of liver tissue in patients with chronic hepatitis B （CHB）. Methods One hundred and one CHB patients were consecutively enrolled in this study, liver tissues and peripheral blood mononuclear cells （PBMCs） were collected. Liver histopathological diagnosis, including fibrosis stage and inflammatory activity grade, was processed by more than two independent pathologists. Total RNA was isolated from PBMCs, levels of ERa mRNA and AR mRNA from PBMCs were measured using real time quantitative polymerase chain reaction （RT PCR）. Results The level of AR mRNA from PBMCs was significantly different among patients with different inflammatory grade and fibrosis stage of liver tissues. Similarly, the level of ERa mRNA from PBMCs was significantly different liver inflammatory grade. Using logistic regression analysis, it is observed that AR mRNA was the principle factor that influence the inflammatory grade of liver tissue, while age and AR mRNA were the principle factors for fibrosis stage of liver tissue in patients with chronic hepatitis CHB. Conclusion The level of AR was an important factor to affect the onset and development progression of CHB patients.

关 键 词：慢性乙型肝炎 雌激素受体α 雄激素受体 肝组织炎症程度分级 肝组织纤维化分期 

分 类 号：R512.62[医药卫生—内科学]