没药甾酮对肝星状细胞HSC-T6增殖的影响  

Effect of guggulsterone on proliferation of hepatic stellate cell line(HSC-T6)

作　　者：贾晓黎[1] 石娟娟[1] 封婷[1] 程延安[1] 潘国英[1] 刘静坤[1] 党双锁[1] 

机构地区：[1]西安交通大学第二医院感染科,710004

出　　处：《肝脏》2013年第8期522-525,共4页Chinese Hepatology

基　　金：陕西省科学技术研究发展计划(2007K16-07(9))

摘　　要：目的研究没药甾酮对肝星状细胞增殖的影响。方法采用MTT法观察不同浓度没药甾酮对肝星状细胞增殖的影响;应用流式细胞术检测细胞周期的变化;化学发光法检测细胞培养液中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)和过氧化氢酶(CAT)水平。结果没药甾酮可显著抑制肝星状细胞生长,并呈时间、剂量依赖,24 h最大抑制率可达(57.4±2.45)%(100μmol/L);50μmol/L和75μmol/L没药甾酮可使G2/M期细胞比例增多,G0/G1和S期比例下降;没药甾酮可使MDA水平下降,GSH和SOD、CAT活性升高。结论没药甾酮可干扰细胞周期,抑制肝星状细胞增殖;还可降低氧化应激反应从而抑制肝星状细胞活化。Objective To investigate the effects of guggulsterone on cell proliferation of hepatic stellate cell line（HSC T6） cultured in vitro. Methods HSC-T6 cells were treated with different concentrations of guggulsterone （5-100μmol/L） for 24 72 hours. Cell proliferation was tested by MTT assay. Cell cycle was investigated using flow cytometer. Superoxide dismutase（SOD）, malondialdehyde（MDA） , glutathione（GSH） and catalas（CA） levels in the culture media were measured by spectrophotometric colorimetry. Results Guggulsterone significantly inhibited HSC T6 cell proliferation in a dose dependent and time-dependent manner, and the maximal inhibition effect was observed at the concentration of 100 /μol/L guggulsterone （57.4±2.45%） in 24 hours. Flow eytometer analysis showed that guggulsterone significantly enhanced G2/M but inhibited G0/G1 and S proportion of cell cycle in response to the treatment of 50 μmol/L and 75μmol/L guggul sterone respectively. Further analysis indicated that 50 μmol/I, and 75 μmol/L guggulsterone significantly decreased the level of MDA but increased the activity of GSH and levels of SOD and CAT in the cuhure medium. Conclusion Gugguls terone can inhibit cell proliferation through interfering with cell cycle in hepatic stellate cells. Moreover, guggulsterone can reduce ocidative stress to inhibit the activation of hepatic stellate cells. Therefore, both of these effects induced by gugguls terone will contribute to the inhibition of liver fibrosis.

关 键 词：没药甾酮 肝星状细胞 氧化应激 细胞周期 

分 类 号：R285[医药卫生—中药学]