自体血注入法制备大鼠脑内囊出血模型的改良与体会  

Improvement and Experience of Autologous Blood into the Legal System for Internal Capsule Hemorrhage Rats Model

作　　者：丁绪平[1] 马风杰[1] 张学敏[1] 赵玉娇[1] 李芳[1] 

机构地区：[1]潍坊医学院神经病学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2013年第4期277-279,I0001,共4页Acta Academiae Medicinae Weifang

摘　　要：目的改良自体血注入法制备大鼠内囊出血模型,并通过多个指标的测定来评价该方法的价值。方法称取体重250~300g雌性Wistar大鼠,分为改良自体血注入前囟前0.5mm组、改良自体血注入前囟后1mm组、改良自体血注入前囟后2mm组、传统自体血注入前囟后2mm组与假手术组。手术后评价包括手术成功率、手术时间、大鼠神经功能缺损评分、大鼠体重、HE染色。结果改良自体血注入前囱后2mm组成功率明显高于其他组,手术时问明显缩短,神经功能评分无明显差异,HE染色出血周围细胞大量坏死、凋亡,炎症细胞大量浸润,脑组织疏松。结论改良自体血注入法制备大鼠内囊出血模型简单、可行,模型成功率较高,是较可靠、理想的内囊出血模型制备方法。Objective To improve autologous blood injection for the legal system of rat with internal capsule hemorrhage model , and to evaluate the value of thismethod through the multiple index measurement .Methods Female Wistar rats weighting between 250g^300g were chosen and divided into four groups：improved autologous blood into the anterior fontanelle before 0.5mm group,improved autolo-gous blood into the anterior fontanelle 1mm behind group,improved autologous blood into the anterior fontanelle 2mm behind group,traditional autologous blood into the anterior fontanelle 2mm behind group and sham-operated group.Surgery evaluation including the success rate of the operation,operation time,rats nerve function defect score ,body weight and HE staining.Results The success rate of improved autologous blood into the anterior fontanelle 2mm behind group was obviously higher than that of the other groups ,operation time was significantly shor-ter,nerve function score showed no obvious difference .HE staining showed hemorrhage surrounding cells apoptosis of necrosis ,a large number of inflammatory cells infiltration,and brain loose.Conclusion Improved autologous blood injection for the legal system of rats with internal capsule hemorrhage model is simple and feasible ,model success rate is higher ,more reliable for the ideal preparation of internal capsule hem-orrhage model.

关 键 词：内囊出血 模型 大鼠 

分 类 号：R651.15[医药卫生—外科学]