止消通脉宁干预TGF-β_1诱导的HK-2细胞VEGF mRNA的表达  

The Effect of Zhixiaotongmaining on the Expression of VEGF mRNA of Human Renal Tubular Epithelial Cell HK-2 Induced by TGF-β_1

作　　者：张玉梅 杨丽霞[2] 程涛[2] 刘铜华[3] 吴丽丽[3] Margetts Peter Joseph 

机构地区：[1]甘肃省兰州市西固区人民医院,兰州730060 [2]甘肃省中医药研究院,兰州730050 [3]北京中医药大学,北京100029 [4]McMaster University

出　　处：《中国中西医结合肾病杂志》2013年第8期674-676,共3页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：科技部国际科技合作项目(No.2009DFA31520);北京中医药大学创新团队项目(No.2011-CXTD-19)

摘　　要：目的:通过观察止消通脉宁药物血清干预转化生长因子-β1(TGF-β1)诱导的人肾小管上皮细胞(HK-2)血管内皮生长因子(VEGF)mRNA的表达,进一步探讨其在防治肾间质纤维化方面的作用。方法:将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养;实验分为6组:空白对照组、单纯TGF-β1诱导组(TGF-β110ng/ml)、空白血清对照组(TGF-β110ng/ml+10%空白血清)、干预1组(TGF-β110ng/ml+10%低剂量止消通脉宁药物血清)、干预2组(TGF-β110ng/ml+10%中剂量止消通脉宁药物血清)、干预3组(TGF-β110ng/ml+10%高剂量止消通脉宁药物血清)。药物干预24h后,荧光定量PCR检测VEGF mRNA的表达。结果:HK-2细胞经TGF-β1诱导后,VEGF mRNA的表达显著上升,与空白对照组相比差异有统计学意义(P<0.05),经止消通脉宁药物血清干预后,VEGFmRNA的表达逐步下降,与单纯TGF-β1诱导组相比差异有统计学意义(P<0.05)。结论:止消通脉宁在一定程度上能够抑制TGF-β1诱导的人肾小管上皮细胞纤维化,其机制的可能与调节纤维化细胞因子VEGF的mRNA表达有关。Through studying the effect of Zhixiaotongmaining （ZXTMN） on the expression of VEGF mRNA of human renal tubular epithelial cells HK - 2 induced by transforming growth factor - 13, （ TGF - 131 ）, to explore the mechanism of ZXT- MN on the prevention and treatment of renal fibrosis. Methods：The HK -2 cells were cultured by DMEM / F12 （ 1： 1 ） with 10 % fe- tal bovine serum and divided into control group, TGF -β1group （TGF- 131 10 ng/ml）, animal blank serum control group（TGF-β1 10 ng/ml ＋ 10% animal serum）, ZXTMN drug -containing serum therapy groups （TGF -β1 10 ng/ml ＋ 10% low dose ZXTMN,TGF -β1 10 ng/ml ＋ 10 % middle dose ZXTMN,TGF - β1 10 ng/ml ＋ 10% high dose ZXTMN）. After 24 h, the expression of VEGF mRNA was tested by fluorescence quantitatiye PCR assay. Results：After the HK -2 cell was induced by TGF - β1, the expression of VEGF mRNA was much increased compared with the control（P 〈0.05）. But the expression of VEGF mRNA was decreased compared with TGF - β1 group （P 〈 0.05 ）. Conclusion：ZXTMN could prevent the development of renal fibrosis to a certain extent.

关 键 词：止消通脉宁 转化生长因子-Β1 人肾小管上皮细胞 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]