Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting  

作　　者：Nadeem Akhtar ABBASI Tariq PERVAIZ Ishfaq Ahmed HAFIZ Mehwish YASEEN Azhar HUSSAIN 

机构地区：[1]Plant Tissue Culture Laboratory,Department of Horticulture,Pir Mehr Ali Shah-Arid Agriculture University

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第9期774-784,共11页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project(No.20-1540/R&D/09)supported by the Higher Education Commission(HEC)of Pakistan

摘　　要：In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%,7%,10%,12%,14% (v/v)) for 12 min and HgCl2 (0.01%,0.05%,0.10%,0.20%,0.25% (w/v)) for 2 min.A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl.Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins,benzylaminopurine (BAP),kinetin,and N6-(2-isopentyl)adenine (2iP).Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots,while the highest shoot length was yielded by the combination of BAP 0.5 mg/L,kinetin 0.5 mg/L,and 2iP 3 mg/L.Higher levels of cytokinins induced callogenesis,vitrification and stunted growth to some extent.For rhizogenesis,uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins.The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl （5%,7%,10%,12%,14% （v/v）） for 12 min and HgCl2 （0.01%,0.05%,0.10%,0.20%,0.25% （w/v）） for 2 min.A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl.Caulogenic response was assessed on Murashige and Skoog （MS） medium fortified with assorted combinations of the cytokinins,benzylaminopurine （BAP）,kinetin,and N6-（2-isopentyl）adenine （2iP）.Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots,while the highest shoot length was yielded by the combination of BAP 0.5 mg/L,kinetin 0.5 mg/L,and 2iP 3 mg/L.Higher levels of cytokinins induced callogenesis,vitrification and stunted growth to some extent.For rhizogenesis,uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins.The best rooting expression was observed with naphthaleneacetic acid （NAA） 1 mg/L combined with indole-3-butyric acid （IBA） 2 mg/L and paclobutrazol （PBZ） 1 mg/L.

关 键 词：Eriobotryajaponica MICROPROPAGATION Sterilization of Ioquat Plant growth regulator Shoot proliferation RHIZOGENESIS 

分 类 号：S667.3[农业科学—果树学]