左西替利嗪对外周血衍生的树突状细胞表达CD86和分泌IL-8的影响  

Effect of levocetirizine on CD86 expression and IL-8 secretion by monocyte-derived dendritic cells

作　　者：王淼淼[1] 张彩萍[2] 钱齐宏[1] 季江[3] 李晶冰[4] 闫桢桢[5] 杨莹[5] 周武庆[5] 崔盘根[5] 

机构地区：[1]苏州大学附属第一医院,苏州215006 [2]江苏大学附属人民医院,镇江212000 [3]苏州大学附属第二医院,苏州215004 [4]徐州市中心医院,徐州221116 [5]中国医学科学院皮肤病研究所,南京210042

出　　处：《中国麻风皮肤病杂志》2013年第9期564-566,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:确定左西替利嗪对组胺诱导的单核细胞衍生的树突状细胞(MoDC)表达CD86和分泌IL-8的影响。方法:分离健康志愿者外周血单核细胞,进行体外诱导培养获得MoDC,以组胺作进一步诱导,并加入左西替利嗪进行干预,以FACS和ELSIA检测MoDC中CD86表达和IL-8分泌。结果:经组胺诱导后,MoDC的CD86表达水平和IL-8分泌量明显增多,左西替利嗪干预后CD86表达和IL^8分泌均受到不同程度的抑制(P<0.05)。此外,不同个体来源的MoDC对药物的敏感性也存在差异。结论:左西替利嗪的抗组胺作用机制是抑制组胺诱导的MoDC表达CD86和分泌IL-8,但临床药物治疗应个体化。Objective： To detect the effects of levocetirizine on the expression of CD86 and production of IL-8 by monocyte-derived dendritic cells （MoDC）. Methods： Monocytes were isolated from healthy volun- teers and were induced into monocyte-derived dendritic cells （MoDC）. MoDC was cultured with histamine and levocetirizine separately. The secretion of CD86 and IL-8 by MoDC in the co-cultures was detected by FACS and ELSIA methods, separately. Results： In the culture with histamine, the secretion of CD86 cells and IL-8 was increased, while in the culture with levocetirizine the secretion of CD86 cells and IL-8 was re- duced （P〈0.05）. However, this suppressive effect varied in different subjects. Conclusions： Levocetirizine can suppress the increase of CD86 cells and IL-8 secretion after histamine stimulation. The clinical treatment with levocetirizine should be individually.

关 键 词：左西替利嗪 树突状细胞 CD86 IL-8 

分 类 号：R96[医药卫生—药理学]