高效液相色谱法测定马尿酸乌洛托品片的有关物质  

Determination of related substance in methenamine hippurate tablets by HPLC

作　　者：海川[1] 何胜利[2] 

机构地区：[1]郑州人民医院颐和医院,河南郑州450000 [2]天津药业集团新郑股份有限公司,河南新郑451150

出　　处：《中国医药科学》2013年第18期118-120,共3页China Medicine And Pharmacy

摘　　要：目的应用高效液相色谱法测定马尿酸乌洛托品片的有关物质。方法采用C18色谱柱(4.6mm×250mm,5μm),甲醇-乙腈-0.1%磷酸溶液(40∶25∶35)为流动相,流速:0.9mL/min,检测波长:217nm。结果在选定的色谱条件下,马尿酸乌洛托品与有关物质分离完全.最低检测限为30ng,精密度良好(RSD=0.78%)。结论该方法简便、准确、专属性好、灵敏度高,可用于马尿酸乌洛托品片的有关物质测定。Objective To determine the related substances of Methenamine hippurate Tablets by HPLC. Methods The separation was performed on Cz8 column（4.6mm 250mm,5 L m）,The mobile phase consisted of methanol and acetonitrile-0.1% phosphoric acid solution （40： 25：35 ）,the flow rate was set at 0.9mL/min with detection at 217nm. Results Under tile established chromatographic conditions, the related substances and methenamine hippurate were separated completely.The least detective range was 30rig.The precision in a day is good（RSD=0.78%）. Conclusion The method is simple,accurate,specific and sensitiveness for the determination methenamine hippurate Tablets related substance.

关 键 词：马尿酸乌洛托品片 有关物质 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]