小麦幼胚愈伤组织的遗传转化  

Transgenosis of callus from wheat's immature embryos

作　　者：程高峰[1] 关占良[1] 

机构地区：[1]河北农业大学教学实验农场,河北保定071001

出　　处：《广东农业科学》2013年第18期1-2,15,共3页Guangdong Agricultural Sciences

摘　　要：将从水稻中提取的抗性调节转录因子OsDREB2.2基因,通过农杆菌介导进入小麦组织细胞内的方法,培育筛选稳定遗传的植株,使小麦中的一系列抗性基因得以表达,从而培育出抗逆性强的小麦新品种。采用河北农业大学自主培育的小麦新品种河农825、河农827、河农831等为试验材料,以各自幼胚为外植体,诱导的愈伤侵染农杆菌后,获得的抗性愈伤组织数平均为170.44、分化愈伤数平均为24.56、抗性芽与转化幼胚总数的比例平均为0.96%。表明本研究中采用的愈伤组织诱导、农杆菌侵染和植株再生条件是适宜的。Resistant regulating transcription factors OsDREB2.2 gene extracted from rice was mediated into wheat tissue cells by agrobacterium tumefaciens, to foster and screen stable genetic plant. Then a series of resistance genes in wheat could express, so as to develop a strong resistance of wheat varieties. Taking new varieties of wheat Agricultural University of Hebei 827, 825 and 831 bred by Agricultural University of Hebei as materials, each embryo was used as explant, after callus infection of agrobacterium, authors obtained 170.44 resistant callus, in the transformation of wheat immature embryo, obainted an average of 24.56 differentiation of callus. Resistant shoots and transformation young embryo percentage of the total reached 0.96%. The resulit indicated that the callus induction, agrobacterium infection and plantlet regeneration condition in this study is suitable.

关 键 词：小麦 愈伤组织 幼胚 

分 类 号：S512.1[农业科学—作物学]