甘蔗试管苗瓶内生根与瓶外生根培养方法比较研究  

Comparative study on in vitro and ex vitro rooting culture of sugarcane microshoots

作　　者：刘红坚 范业赓[1,2] 何为中 莫磊兴[1,2] 王天顺[1,2] 牙禹[1,2] 

机构地区：[1]广西壮族自治区农科院甘蔗研究所/中国农科院甘蔗研究中心/广西甘蔗遗传改良重点实验室,广西南宁530007 [2]农业部甘蔗品质监督检验测试中心(南宁),广西南宁530007

出　　处：《广东农业科学》2013年第18期13-15,共3页Guangdong Agricultural Sciences

基　　金：省级公益性科研院所基本科研业务费项目(G2010011);广西甘蔗研究所基本科研业务专项(G2010007);广西科学研究与技术开发项目(桂科合140011-3);广西重点实验室建设项目(12-A-04-01);广西农业科学院基本科研业务专项(桂农科2012YM10;2012YM11)

摘　　要：以新台糖22号(ROC22)甘蔗无根试管苗和有根试管苗为材料,对其移栽苗圃后成活率、分蘖率、株高、根系生长情况及两种试管苗生根成本进行了研究。结果表明:两种生根法各有优劣;传统的瓶内生根技术有利于组培苗的生根,提高了苗的生根率、长势和移栽成活率及苗的质量;瓶外直接生根则有利于降低甘蔗组培的生产成本,提高组培苗的产量和缩短苗的生产周期。The survival rate, tillering rate, plant height, growth of root system and rooting cost of in vitro rooting and ex vitro rooting of sugareane（ROC22）microshoots which were transplanted in green house were studied. The result showed that the two rooting methods of microshoots had its own advantages respectively. The traditional in vitro rooting was beneficial to rooting of microshoots, and could enhance rooting rate, growth, transplant survival rate and quality of seedling. However, ex vitro rooting of microshoots was propitious to reduce cost of production, enhance number of mieroshoots, reduce time of production cycle.

关 键 词：甘蔗 试管苗 瓶内生根 瓶外生根 比较研究 

分 类 号：S566.1[农业科学—作物学]