紫茎泽兰提取物的体外抗菌活性研究  

Antibacterial activity in vitro of Eupatorium adenophorum extracts

作　　者：池水晶 宝珠鸣[2] 王艳红[1] 罗夙医[1] 李玉鹏[3] 宝福凯[1] 柳爱华[1] 

机构地区：[1]昆明医科大学热带医学研究所,云南昆明650031 [2]昆明医科大学护理学院,云南昆明650031 [3]昆明医科大学药学院,云南昆明650031

出　　处：《广东农业科学》2013年第18期73-74,107,共3页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(81060134);云南省自然科学基金(2007C069M;2010CD221;2011FB244)

摘　　要：探讨紫茎泽兰(Eupatorium adenophorum)95%乙醇、石油醚、乙酸乙酯、正丁醇、甲醇5种粗提物的体外抗菌效果。通过倍比稀释法分析紫茎泽兰5种提取物对大肠埃希氏菌、金黄色葡萄球菌、痢疾杆菌和白色念珠菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。试验结果表明:紫茎泽兰乙酸乙酯粗提物的抑菌效果最显著,当其浓度为12.5 mg/mL时,对大肠杆菌、金黄色葡萄球菌、痢疾杆菌有明显的抑制作用,当其浓度为6.25 mg/mL时,对白色念珠菌的抑菌效果明显。紫茎泽兰5种粗提物对白色念珠菌的抑菌作用均大于对大肠杆菌、金黄色葡萄球菌、痢疾杆菌的抑菌作用。说明紫茎泽兰5种粗提物对人类常见致病菌有明显的抑制和杀灭作用,在抑菌方面有一定的价值.To investigate the antibacterial activity in vitro of Eupatorium adenophorum extracts, authors used 95% ethanol, petroleum ether, ethyl acetate, n-butanol and methanol to extract the antibacterial ingredients. The antibacterial activities （MBC and MIC） were analyzed with Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Candida a/b/carts by double dilution method. The result showed that E. adenophorum extracts used by ethyl acetate had the significant antibacterial effect on E. coli, S. aureus and S. dysenteriae, when its concentration was 12.5 mg/mL. When its concentration was 6.25 mg/mL, it could significantly suppress C. albicans. 5 E. adenophorum extracts had higher suppression effect on C. albicans than E. coli, S. aureus and S. dysenteriae. The result indicated that 5 E. adenophorum extracts could significantly inhibit and kill common human pathogens. Therefore, the extracts from E. adenophorum may have a certain development value in antibacterial aspects.

关 键 词：紫茎泽兰 人类常见致病菌 倍比稀释法 抗菌作用 

分 类 号：S482.292[农业科学—农药学] R282.71[农业科学—植物保护]