番茄果实颜色相关MYB转录因子的基因表达研究  

Research on gene expression of MYB transcription factor related to tomato fruit color

作　　者：陈云云[1,2,3] 罗静瑶[2,3] 赵惠[2,3] 穆雷[2,3] 谢俊[2,3] 韦双双[3] 夏幽泉[3] 汤华[1,2,3] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228 [2]海南大学海南热带生物资源可持续利用国家重点实验室培育基地,海南海口570228 [3]海南大学农学院,海南海口570228

出　　处：《广东农业科学》2013年第18期127-129,F0002,共4页Guangdong Agricultural Sciences

基　　金：教育部高等学校博士点科研基金(20104601120001);热带作物种质资源保护与开发利用教育部重点实验室开放课题(2010hckled-01);海南省自然科学基金(311025);海南大学青年基金(qnjj1213)

摘　　要：在番茄果实颜色发育过程中,转录因子参与其中。在源于番茄野生种Solanum lycopersicoides的导入系群体中,发现了1个黄果突变体,以红果亲本番茄及该黄果突变体为遗传材料,通过数字基因表达谱测序及RT-PCR,对MYB转录因子的表达模式进行研究。筛选到多个与果实颜色相关的差异表达MYB转录因子,并进行了半定量RT-PCR验证。During the tomato fruit color development, transcription factor may play important roles. From an introgression line population derived from tomato wild species Solarium lycopersicoides, we found a yellow-fruit mutant line which was different from red- fruit patent. We took red-fruit parent tomato and yellow-fruit mutant as genetic materials to study the gene expression profiles of MYB transcription factor by digital gene expression sequencing and RT-PCR. As resuhs, we screened out many MYB transcription factors related to tomato fruit color and differential expressed in yellow-fruit and red-fruit tomato, and verified one of them by RT-PCR.

关 键 词：番茄 果实颜色 黄果突变体 MYB转录因子 基因表达 

分 类 号：Q786[生物学—分子生物学]