塔胞藻及海水小球藻对草甘膦致毒胁迫的生理应答  

Physiological Response of Algae Pyramidomonas delicatula and Chlorella saccharophila to Glyphosate Toxicity Stress

作　　者：田密[1] 郑贝贝[2] 苏金丽[2] 宋秀梅[2] 王洪斌[2] 

机构地区：[1]连云港市海州实验中学,江苏连云港222023 [2]淮海工学院海洋学院,江苏连云港222005

出　　处：《水产科学》2013年第9期541-544,共4页Fisheries Science

基　　金：科技部"十二五"科技支撑项目(2011BAD13B03);江苏省科技厅苏北科技发展计划项目(BN2011035)

摘　　要：为提示农药污染对水环境破坏,揭示海洋微藻类作为环境毒理学评价指标的潜在应用,研究了塔胞藻、海水小球藻的生长及叶绿素a合成对除草剂草甘膦的致毒胁迫生理应答。试验结果表明,培养前3d,在试验质量浓度范围内,草甘膦对塔胞藻生长均无抑制作用,塔胞藻对10μL的草甘膦呈致毒兴奋效应,最高值出现在第3d,比对照组高39%;自第4d开始,草甘膦对塔胞藻呈明显抑制作用,并呈明显的剂量关系,抑制作用在80μL试验组、培养至7d时达最高,试验组生物量为对照组的47%;草甘膦对海水小球藻的致毒胁迫作用明显强于对塔胞藻,培养第2d开始就表现出明显致毒胁迫效应,随草甘膦的质量浓度增加及培养时间的延长,抑制作用增强,增强趋势非常明显,培养至第7d,10、20、40、60、80μL试验组生物量分别为对照组的27.4%、24.6%、23.7%、21.9%和13%。草甘膦对塔胞藻、海水小球藻叶绿素a含量均表现抑制效应,最小值出现在80μL试验组,叶绿素a的含量仅为对照组的24.3%和7.54%,对海水小球藻的致毒胁迫效应远大于塔胞藻。表明海水小球藻可作为检测水域环境草甘膦污染的毒理学指标。The physiological effects of herbicide glyphosate on growth and synthesis of chlorophyll a were studied in algae Pyramidomonas delicatula and Chlorella saccharophila to alert the pesticide pollution severity in water environment and to reveal potential application of marine microalgae in environmental toxicological evaluation. The results showed that the glyphosate had no inhibitory effect on the alga growth within the concentration in all experimental groups in the first 3 days, showing toxicity excitement at 10/μL of glyphosate, with the maximal value in 3 day, 39% higher than that in control group. From the day 4, however, glyphosate showed significant inhibitory effect on P. delicatula and a clear dosedependent relationship, up to the peak in 80 μL group 7 d after cultivation, the biomass in the experimental group representing 47 % of the control group. Glyphosate was found to have stronger toxicity stress on C. saccharophila than on P. delicatula, being increased with the increase in concentration and time elapse from the 2 d. In 7 day cultivation, the biomass was found to be 27.4%of the control group at 10 bd. of glyphosate, 24.6% at 20 /μL of glyphosate, 23.7% at 40 μL of glyphosate, 21.9% at 60 μL of glyphosate, and 13% at 80μL. of glyphosate. The glyphosate showed inhibitory effect on chlorophyll a of the algae, the minimal value in 80 μL group, and the content of chlorophyll a was only 24. 3% （P. delicatula） and 7.54%（C. saccharophila） of the control group. Glyphosate showed much more toxicity stress on C. saccharophila than on P. delicatula, indicating the feasibility of C. saccharophila as the toxicological evaluation for glyphosate water pollution.

关 键 词：草甘膦 塔胞藻 海水小球藻 致毒胁迫 生理应答 

分 类 号：Q89[生物学]