松鼠葡萄球菌抑制屋尘螨引起的混合型气道炎症反应  

Staphylococcus sciuri suppressed mixed allergic airway inflammation induced by house dust mite extract in an experimental mouse model

作　　者：赵敏[1,2] 李智[3] 于永春[4,5] 陈春球[4,5] Holger Garn Harald Renz 

机构地区：[1]上海市第十人民医院检验科 [2]同济大学附属第十人民医院检验科,上海200072 [3]上海市杨浦区中心医院检验科,上海200090 [4]上海市第十人民医院中心实验室 [5]同济大学附属第十人民医院中心实验室,上海200072 [6]德国马尔堡大学,德国马尔堡35039

出　　处：《国际检验医学杂志》2013年第17期2224-2226,2228,共4页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(81170345)

摘　　要：目的探讨松鼠葡萄球菌对屋尘螨(HDM)致敏的小鼠哮喘模型的影响作用。方法 BALB/c小鼠于实验第0、7、14、21天接受经鼻腔内注射PBS液或HDM提取液致敏。松鼠葡萄球菌的"接种"始于致敏前9d,每周3次,止于实验第20天。于末次致敏后24h和48h分别处死小鼠。对支气管肺泡灌洗液(BAL)进行细胞计数和分类;ELISA法检测BAL中CXCL1、CXCL2、CCL11(eotaxin)、IL-5、IFN-γ、IL-10和IL-17的含量及血清总IgE,HDM特异性IgG1、IgG2a;流式微球技术(CBA)检测体外培养淋巴分泌液中细胞因子IL-4、IL-5、IL-13、IFN-γ、IL-10、IL-17A和GM-CSF的含量;采用实时荧光定量PCR检测肺组织中相关基因mRNA表达;并对肺组织PAS染色后进行病理组织学观察。结果松鼠葡萄球菌抑制了HDM抗原诱导的混合型气道炎症(以中性粒细胞和嗜酸性粒细胞为主);降低了BAL中CXCL1和IL-5的蛋白水平;阻止体外培养淋巴细胞分泌IL-5、IFN-γ、IL-10和IL-17等因子;并减轻了高脚杯细胞增生(GCH)和黏液分泌增多等组织学改变。结论 "接种"松鼠葡萄球菌对HDM致敏的小鼠模型具有保护效应。Objective To explore the influential role of Staphylococcus sciuri（S.sciuri）in an experimental murine asthma model sensitized by HDM allergen.Methods BALB/c mice received an intranasal instillation of PBS or HDM extract（50μg or 100μg）on days 0,7,14,21.Nine days before the first sensitization,the mice were given an inoculation of 108 CFU/25μL Staphylococcus sciuri.The inoculation took place for 3times in one week and stopped on day 20.24and 48hours after the final sensitization,the mice were sacrificed respectively.Cell count and differentiation were made for bronchoalveolar lavage fluid（BAL）cells;protein contents of CXCL1,CXCL2,CCL11（eotaxin）,IL-5,IFN-γ,IL-10and IL-17in BAL and the amount of HDM-IgG1,HDM-IgG2aand total IgE in the serum were measured by enzyme-linked immunosorbent assay（ELISA）;the levels of cytokines including IL-4,IL-5,IL13,IFN-γ,IL-10,IL-17Aand GM-CSF in in-vitro restimulated lymphocytes were detected by Cytometric Bead Array（CBA）;mRNA expressions of specific genes were determined by real time PCR;lung tissues were PAS-stained for histological examinations.Results S.sciuri inhibited the mixed airway inflammation of neutrophils and eosinophils,lowered the protein levels of CXCL1and IL-5in the BAL,prevented the secretions of IL-5,IFN-γ,IL-10and IL-17from restimulated lymphocytes and altered histological changes including goblet cell hyperplasia（GCH）and mucus hypersecretion in a mouse model which was sensitized by HDM allergen.Conclusion Inoculation of S.sciuri exerted a protective effect on HDM-induced mouse model.

关 键 词：尘螨科 葡萄球菌属 支气管肺泡灌洗液 

分 类 号：R562.25[医药卫生—呼吸系统]