男性尿道口细胞HPV感染基因型分布的研究  

A study of HPV infective genotypes distribution in urethral mouth cells of male cases

作　　者：夏思钧[1] 龚培尧[2] 耿建祥[2] 平春敏[2] 靳春萍[2] 龙秀荣[2] 施启丰[2] 夏林[2] 王宏景[2] 王晓红[2] 

机构地区：[1]江苏省射阳县人民医院病理科,江苏盐城224300 [2]南京中医药大学附属第三医院病理科HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2013年第17期2231-2233,共3页International Journal of Laboratory Medicine

基　　金：南京市卫生局中医专项支助项目(2009-92)

摘　　要：目的探讨男性尿道口细胞中人乳头瘤病毒(HPV)不同基因型别的感染情况及其临床意义。方法从304例男性尿道口细胞标本中提取23种HPV DNA,采用基因扩增结合基因芯片杂交技术对其进行HPV基因检测,并对受检者行基因型别的分析。结果 304例男性受检者中检出HPV感染者117例,总阳性率为38.49%(117/304)。其中单一型别的HPV感染者75例,占24.67%(75/304),单一型别的感染中,HPV6型感染者26例,阳性率为34.67%(26/75),是单一型别最主要的感染类型,其次分别为HPV11型10例、16型7例、58和81型各6例、33型5例,感染率分别为13.33%(10/75)、9.33%(7/75)、8.00%(6/75)、8.00%(6/75)、6.67%(5/75)。多型HPV感染者42例,占13.82%(42/304),其中二重感染者34例,占多型感染的80.95%(34/42),三重感染者6例,占多型感染的14.29%(6/42)。HPV感染的年龄组分布比较差异无统计学意义(P>0.05)。结论 HPV 6型、11型、16型、58型、81型、33型是男性尿道口细胞感染的主要基因型别,基因扩增结合基因芯片检测技术是一种比较适合临床对男性进行HPV分型检测的敏感性高、特异性强的诊断方法,尤其适合开展男性尿道口细胞HPV感染的分子流行病学的研究。Objective To study the genotypes of human papillomavirus（HPV）and its clinical significance in urethral mouth cells of male genitalia.Methods Polymerase chain reaction（PCR）and gene-chips technology were utilized for the detection of 23kinds of HPV genotypes in urethral mouth cells from 304male cases and the related HPV genotypes of 304cases were analyzed.Results In 304male subjects,HPV positive were 117cases,total infection rate of HPV was 38.49%（117/304）.single genotypes were 75cases,the infection rate of single genotypes was 24.67%（75/304）,the predominant type of single infection with HPV was HPV 6（34.67%,26/75）,Followed By HPV11（13.33%,10/75）,HPV16（9.33%,7/75）,HPV58and 81（8.00%,6/75）,HPV33（6.67%,5/75）,respectively.The infection rate of multiple genotypes was 13.82%（42/304）,the infection rate of two genotypes was 80.95%（34/42）and the infection rate of three genotypes was 14.29%（6/42）in multiple genotypes.Age distribution of HPV infection showed no statistically significant（P0.05）.Conclusion HPV 6、11、16、58、81、33types were the main genotypes in urethral mouth cells from 304male cases.PCR and gene-chip technology can detect single and multiple HPV genotypes in cell samples of male urethral mouth with high sensitivity and specificity,especially fit for study on molecular epidemiology of HPV infection in cells of male urethral mouth.

关 键 词：乳头状瘤病毒科 尿道 基因型 

分 类 号：R695[医药卫生—泌尿科学]