临床用血前交叉配血不合104例原因分析  

Analysis of the causation of the 104 samples cross-matching incompatibility before blood transfusion

作　　者：吴学忠[1,2] 吕蓉[1,2] 李敏[1,2] 李素萍[1,2] 於娟[1,2] 王超[1,2] 盛琪琪[1,2] 王伦善[1,2] 

机构地区：[1]合肥市中心血站 [2]安徽省血液中心,安徽合肥230031

出　　处：《国际检验医学杂志》2013年第17期2263-2264,共2页International Journal of Laboratory Medicine

摘　　要：目的分析总结临床输血中遇到的疑难交叉配血病例,探讨输血前交叉配血不合的原因,使临床输血更加的科学、安全、有效。方法回顾性分析合肥市不同医院送到本站进行用血前疑难交叉配血病例104例,疑难交叉配血采用盐水法、MP法和抗人球蛋白法等方法检测。结果 104例疑难交叉配血标本中,ABO血型正、反定型不符1例,受血者不规则抗体阳性28例,冷凝集素凝集5例,红细胞直接抗人球蛋白试验阳性43例。104例疑难交叉配血结果为,盐水法交叉配血主/次出现凝集13例,聚凝胺法交叉配血主/次侧出现凝集34例,抗人球蛋白法交叉配血主/次侧出现凝集44例。结论交叉配血不合的原因主要有:缗钱状假凝集,冷凝集素所致非特异性凝集,红细胞被致敏引起的凝集,不规则抗体引起的凝集等。临床用血前交叉配血应选择能同时检测完全抗体和不完全抗体的方法,确保患者输血安全。Objective To summative analyze the suspicious cross-matching met in clinical blood transfusion and to explore the reasons of cross-matching incompatibility before blood transfusion,and ensure the clinical transfusion more scientific,safe and effective.Methods 104suspicious cross-matching cases met in blood Center of Hefei were retrospectively analyzed by using brine,MP and anti-human globulin methods.Results In 104suspicious cross-matching cases,including one case positive and reverse ABO blood group inconformity,28cases irregular antibody positive,5cases cold agglutinin,43cases red blood cell direct anti-human globulin testing positive.In 104cases suspicious cross-matching cases,including 13cases major/minor agglutination with brine method,34cases major/minor agglutination with polybrene method,44cases major/minor agglutination with anti-human globulin method.Conclusion The mostly reasons of the cross-matching incompatibility have the false agglutination of cord-like money,the non-specific agglutination due to cold agglutination,the agglutination due to red blood cells sensitived,the agglutination due to irregular antibody.The experimentation of the full antibodies and incomplete antibodies should be detected simultaneously before before the clinical blood transfusion,and ensured the patients safe about blood transfusion.

关 键 词：血型鉴定和交叉配血 不规则抗体 输血 

分 类 号：R446.6[医药卫生—诊断学]