83例疑难ABO血型鉴定及分析  

Identification and analysis of the 83 cases difficult ABO blood group

作　　者：胡晓玉[1,2] 吴学忠[1,2] 吕蓉[1,2] 李敏[1,2] 李素萍[1,2] 於娟[1,2] 王超[1,2] 盛琪琪[1,2] 王伦善[1,2] 

机构地区：[1]合肥市中心血站 [2]安徽省血液中心,安徽合肥230031

出　　处：《国际检验医学杂志》2013年第18期2396-2397,共2页International Journal of Laboratory Medicine

摘　　要：目的检测和鉴定ABO疑难血型,为安全输血提供质量保障。方法采用血型血清学方法进行盐水介质正、反定型;对盐水介质正、反定型不合者使用聚凝胺法检测和不规则抗体检测;采用吸收试验和放散试验进行ABO血型的检测,以准确鉴定ABO疑难血型。结果 83例标本ABO血型检测结果为,AB亚型9例、B亚型1例、弱A抗原7例、弱B抗原6例、低抗-A效价2例、低抗-B效价3例、不规则抗体阳性7例、无抗体O型4例、低抗-B效价O型2例、无抗-B抗体A型5例;37例标本经盐水介质和Polybrine法检测,正、反定型结果符合。结论用聚凝胺法、吸收试验和放散试验进行ABO疑难血型检测,能准确、及时鉴定ABO疑难血型。Objective To detection and identification the difficult ABO blood group,and to provide quality assurance for safe blood transfusion.Methods The positive and reverse ABO blood group was detected by using serological test of blood typing.The positive and reverse ABO blood group inconformity and irregular antibodies were detected by using polybrene method.The difficult ABO blood group was identified accurately by the absorption and elution testing.Results The 83specimens difficult ABO blood group had been detected,including nine cases AB subtye,one case B subtype,seven cases weak A antigen,six cases weak B antigen,two cases low anti-A titer,three cases low anti-B titer,seven cases irregular antibodies,four cases no antibody O type,two cases low anti-B titer O type,five cases no anti-B A type,the positive and reverse ABO blood groups of the 37specimens were conformity by the saline medium and Polybrene method.Conclusion The difficult ABO blood group could be identified accurately and timely by the Polybrene method,absorption testing and elution testing.

关 键 词：ABO血型系统 不规则抗体 血型鉴定 

分 类 号：R446.6[医药卫生—诊断学]