基于透明质酸及硫堇层层自组装的前列腺特异性抗原免疫传感器的研究  

A New Amperometric Immunosensor Based on Layer-by-layer Self-assembly of Hyaluronic Acid and Thionine for Determining Prostate Specific Antigen

作　　者：刘新露[1,2] 袁若[2] 柴雅琴[2] 

机构地区：[1]四川理工学院化学与制药工程学院,四川自贡643000 [2]西南大学化学化工学院,重庆400715

出　　处：《分析测试学报》2013年第9期1081-1085,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20675064)

摘　　要：将带负电荷的透明质酸（HA）和带正电荷的电子媒介体硫堇（Thi）层层自组装到壳聚糖修饰的玻碳电极表面，利用硫堇上的氨基固定纳米金（nano-Au）并固载前列腺特异性抗体（anti-PSA），制得新型前列腺特异性抗原电流型免疫传感器。通过循环伏安法（CV）、微分脉冲伏安法（DPV）考察了该免疫传感器的电化学特性，并对影响该免疫传感器性能的各种因素进行详细研究。在优化实验条件下，此免疫传感器的线性范围为 0.5-4 μg/L 和 4-25 μg/L，检出限为 0.2 μg/L。该免疫传感器具有制备简单、灵敏度高、稳定性好等优点。The preparation and performance of an amperometric immunosensor,based on the use of layer-by-layer self-assembly technique,for the specific detection of prostate specific antigen（PSA） was reported. The immunosensor was developed by immobilizing the anti-PSA through layer-by-layer self-assembly of hyaluronic acid（HA）,thionine（Thi） and gold nanoparticles（nano-Au） on chitosan（Cs） modified glassy carbon electrode（GCE）.The bovine serum albumin（BSA） was employed to block the possible remaining active sites of the nano-Au monolayer,avoiding the non-specific adsorption.The electrochemical characteristics of the immunosensor were studied by cyclic voltammetry（CV） and differential pulse voltammetry（DPV）.The factors influencing the performance of the immunosensor were studied in detail.Under the optimized conditions,the linear ranges of the immunosensor were from 0.5 μg/L to 4 μg/L and from 4 μg/L to 25 μg/L with correlation coefficients more than 0.993,and a detection limit of 0.2 μg/L（estimated to be 3× the standard deviation of zero-dose response）.The practical utilization of the immunosensor has been demonstrated through the determination of PSA in human serum samples.The results determined with the proposed immunosensor agreed fairly well with those obtained by the enzyme linked immune-sorbent assay（ELISA） technique.

关 键 词：透明质酸 硫堇 层层自组装 前列腺特异性抗原 免疫传感器 

分 类 号：O657.1[理学—分析化学] S852.43[理学—化学]