江西省烟草青枯菌生理小种及生化型鉴定  

Identification of Race and Biovar of Ralstonia solanacearum from Tobacco in Jiangxi Province

作　　者：周泽科[1] 张超群 蒋军喜[1] 胡长志[1] 黄婷[1] 

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]江西烟叶科学研究所,江西南昌330025

出　　处：《江西农业大学学报》2013年第4期738-742,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31160358);江西省烟草专卖局(公司)资助项目(201001021)

摘　　要：为给江西烟草青枯病抗性品种的选育和利用提供理论依据,对江西主要烟区青枯病菌(Ralstonia solanacearum)的生理小种和生化型进行了鉴定。从石城、瑞金、会昌、信丰、广昌、峡江6个主要产烟县(市)采集青枯病感病烟株,通过平板稀释分离法从中分离获得24个具有致病性的烟草青枯病菌菌株。选取其中具有代表性的6个菌株,采用鉴别寄主接种、烟叶浸润反应和黑色素产生试验的方法,对其进行生理小种鉴定,结果 6个菌株均属于生理小种1;测定菌株对三种双糖和三种己醇的利用能力以及对硝酸盐还原作用,结果发现6个菌株均属于生化型Ⅲ-1。In order to provide theoretical basis for breeding and utilizing bacterial wih-resistant lobacco varieties,race and biovar of Ralstonia solanacearum in tobacco in Jiangxi Province were identified. Diseased plant samples of tobacco bacterial wilt were collected from six counties of Shicheng, Ruijin, Huichang, Xinfeng, Guangchang and Xiajiang in Jiangxi Province, and 24 strains of the pathogen, Ralstonia solanacearum, were ob- tained from these samples by using the dilution plate isolation method. Six representative strains of them were chosen for race and biovar determination. The experimental results of differential host assay, infiltration reaction and melanin formation showed that all of the six strains belonged to race 1, and on the basis of their capacity in utilizing three disaccharides and three hexanol, as well as their reduction ability to KNO3 , the six strains were also classified as bioval Ⅲ -1.

关 键 词：烟草 青枯劳尔氏菌 生理小种 生化型 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]