基于ITS2序列的黄连及其伪混品的分子鉴定  

Identification of Rhizoma Coptidis and Its Adulterants Based on ITS2 DNA Barcode

作　　者：孙涛[1,2] 孔德英[1,2] 滕少娜[1,2] 陆丽华[1,2] 邓朝晖[1,2] 李应国[1,2] 

机构地区：[1]重庆出入境检验检疫局,重庆400020 [2]国家中药材物种鉴定及质量安全检测重点实验室,重庆400020

出　　处：《贵州农业科学》2013年第9期20-22,共3页Guizhou Agricultural Sciences

基　　金：质检公益性行业科研专项"中药材进出口贸易问题及对策研究"(201010045)

摘　　要：为建立重要中药材黄连及其伪混品的DNA条形码鉴定方法,采用国际通用的条形码序列ITS2对黄连属3个物种6份样品的ITS2序列进行PCR扩增与测序,同时从GenBank下载黄连及其常见混伪品共8个物种18个样本的序列信息。采用MEGA5.05计算黄连及其伪混品的种内、种间K2P距离,并基于K2P模型构建NJ树。结果显示:ITS2种间遗传距离大于种内遗传距离,黄连属及其伪混品不同种样品分别聚类在一起,能很好地区分开来。故ITS2序列可以作为黄连及其伪混品鉴定用的条形码。To build a DNA barcode identification method of the vital Chinese medicine rhizome coptidis and adulterats, PCR amplification and sequencing for ITS2 sequences of six samples in three species were conducted, together with its adulterants downloaded from GeneBank, information of 18 ITS2 sequences for eight species were collected. The genetic distances were computed using MEGAS. 05 in accordance with the Kimura 2-Parameter （K2P） model, and the phylogenetic tree was constructed by neighbor-joining （NJ） method. The results showed the interspecific genetic distance was higher than that of the intraspecific genetic distance, each species clustered into one clade. Therefore, the results indicate that ITS2 is useful to identify rhizoma coptidis from its adulterants.

关 键 词：黄连 DNA条形码 ITS2 鉴定 

分 类 号：Q754[生物学—分子生物学]