镉胁迫下蓖麻相关基因表达的mRNA差异显示  

mRNA Differential Display of Ricinus communis L. Gene Expression Related to Cadmium Stress

作　　者：刘义富[1,2] 毛昆明[2] 

机构地区：[1]丽江师范高等专科学校生命科学系,云南丽江674100 [2]云南农业大学资源与环境学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2013年第5期682-686,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省教育厅科学研究基金项目(2010Y073);云南省环境工程创新人才联合培养地项目(A3003015);鲁地拉水电站土地评价项目(fKX1320511)

摘　　要：通过对100 mg/L镉(Cd)溶液和蒸馏水培养下的蓖麻幼苗mRNA差异显示的前期研究,结果表明:采用百泰克公司生产的通用植物总RNA提取试剂盒能有效地提取蓖麻叶片总RNA,通过3'端锚定引物与随机引物构成的引物对cDNA的PCR扩增,其产物的凝胶电泳共获得11条差异条带,均出现在Cd胁迫下的蓖麻中,说明100 mg/L Cd处理的蓖麻叶片在mRNA水平上发生了明显的变化,可能激发了与Cd耐性和积累相关基因的表达,其产物可能与缓解Cd对蓖麻造成伤害有关。对差异片段的进一步测序、克隆等研究,有助于蓖麻Cd耐性和积累的分子机制研究。In this paper, the difference of cadmium （Cd） resistance and accumulation related genes fragments were investigated by using mRNA differential display technology in Ricinus communis L. , which treated by 100 mg/L Cd （treatment） and distilled water （CK）, respectively. The results showed that total RNA was effectively extracted from the leaf use RNA Isolating Reagent Kit produced by Bioteke Corporation. Altogether 11 differential bands were only obtained in the treatment by PCR which amplified with random constitution between 3＇ anchored primers and random primers. This indicated that the gene expression in mRNA of R. communis L. could be changed by 100 mg/L Cd. It is mainly due to that changed expression product could reduce the damage from Cd. The results can be reference for studies of the molecular mechanism of cadmium tolerance and accumulation

关 键 词：蓖麻 MRNA差异显示 镉胁迫 

分 类 号：X503.231[环境科学与工程—环境工程]