反相高效液相色谱法测定O/W型二氢青蒿素脂肪乳剂中二氢青蒿素含量  

RP-HPLC Determination of Dihydroartemisinin Content in Oil/Water Dihydroartemisinin Lipid Emulsion

作　　者：王荧[1] 马玉樊[1] 卢婷利[1] 陈涛[1,2,3] 

机构地区：[1]西北工业大学生命学院,陕西西安710072 [2]陕西脂质体工程技术研究中心,陕西西安710075 [3]西安力邦制药有限公司,陕西西安710075

出　　处：《中国药业》2013年第18期46-48,共3页China Pharmaceuticals

基　　金：西安高新区创新药物孵化及产业化转化综合平台项目;项目编号:2011ZX 09401-308;西北工业大学研究生创业种子基金;项目编号:Z2010197

摘　　要：目的建立测定O／W型二氢青蒿素脂肪乳剂中二氢青蒿素含量的高效液相色谱法。方法采用AgilentHC—C18柱（250mm×4．6mm，5μm），流动相为乙腈-水（60：40），流速1．0mL／min，柱温25℃，检测波长216nm。结果该方法试样质量浓度0．002129-2．18g／L范围内与检测峰峰面积呈良好线性关系，平均回收率为95．33％，RSD=1．40％（n=9）。结论该方法简便、精密，回收率高，重现性好，可用于二氢青蒿素脂肪乳剂中二氢青蒿素含量的测定。Objective To establish a HPLC method to determine the content of dihydroartemisinin（DHA） in Oil/Water DHA Lipid Emulsion. Methods The Agilent HC C18 column （250 mm ×4.6mm,5μm） was adopted,the mobile phase was acetonitrile-water （60：40）,tl4e flow rate was set at 1.0 mL/min,the column temperature was 25 ℃ and the wavelength was 216 nm. Results The mass concentration of DHA in the range of 0.002 129-2. 18 mg/mL at the detection wavelength of 216 nm had good linear correla- tion with the area of the test peak,the average recovery rate was 95.32% （RSD = 1.2）. Conclusion This method is simple and pre- cise with high recovery rate,high accuracy and good reproducibility,which can be used for the determination of the DHA content in Oil/Water DHA Lipid Emulsion.

关 键 词：反相高效液相色谱法 O W型 二氢青蒿素脂肪乳剂 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286[医药卫生—中医学]