5种旋毛虫肌肉幼虫囊包玻片标本制作方法的比较  

Comparison of Five Methods on Preparing Specimens of Encapsulated Trichinella Spiralis Larvae

作　　者：刘润芳[1] 张小娟[1] 王新彩[1] 祝晓莹[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003

出　　处：《河南科技大学学报（医学版）》2013年第3期163-165,共3页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的寻找快速、简便、有效的旋毛虫肌肉幼虫囊包玻片标本的制作方法。方法采用3种不同浓度醋酸卡红染色法、明矾卡红染色法、不染色共5种方法制作旋毛虫肌肉幼虫囊包玻片标本。结果 10%醋酸卡红染色速度快,囊包轮廓分明,囊内幼虫清晰透明;45%醋酸卡红原液稀释100倍染色,着色较淡,囊包和囊内幼虫清晰可见,且接近不染色。结论旋毛虫肌肉幼虫囊包永久性玻片标本的制作可采用10%醋酸卡红染色法或45%醋酸卡红原液释100倍染色法。Objective To find fast, simple and effective method of encapsulated Trichinella spiralis. Methods Three different acetate-carmine methods, alum-carmine staining method and unstaining method were used to prepare specimens of encapsulated Trichinella spiralis. Results 45% acetate-camine diluted 100 times to stain specimens,the specimens＇ s color was lighter. 10% acetate-camine staining method was fast method. Using the two methods, the specimens had clearly defined capsule edgs and encapsulated larvae were more transparent and readily visible. Conclusion 45% acetate-camine diluted 100 times and 10% acetate-camine staining method can be used to prepare permanent specimens of encapsulated T. spiralis larvae.

关 键 词：旋毛虫 囊内幼虫 醋酸卡红染色 钾明矾染色 

分 类 号：R382.4[医药卫生—医学寄生虫学]