带有IL-24的腺病毒扩增及其在小鼠树突状细胞中的表达  

Amplification of recombinant adenovirus with IL-24 gene and its expression in mouse bone marrow-derived dendritic cells

作　　者：许云树[1] 蒋晓丽[1] 潘巍巍[1] 

机构地区：[1]嘉兴学院医学院生物教研室,浙江嘉兴314001

出　　处：《四川生理科学杂志》2013年第3期99-102,共4页Sichuan Journal of Physiological Sciences

基　　金：浙江省自然科学基金(编号:LQ13H160020;Y2110873);嘉兴市科技局科研项目(编号:2008AY2039-3;2011AY1048-6);浙江省大学生科技创新计划

摘　　要：目的:在293细胞中扩增带有IL-24的腺病毒,感染小鼠树突状细胞,观察IL-24在树突状细胞中的表达。方法:将构建的重组腺病毒表达载体IL-24转染到293细胞中包装、扩增,感染分离培养的小鼠树突状细胞,RT-PCR、Western blot、荧光显微镜检测IL-24的表达。结果:获得了大量的带有IL-24的腺病毒,成功的感染小鼠树突状细胞,RT-PCR和Western blot检测结果显示,IL-24在树突状细胞中高表达。结论:带有IL-24的腺病毒可以高效的感染小鼠树突状细胞。Objective： To amplificate the recombinant adenovirus carrying IL-24 gene, transfect mouse bone marrow-derived dendritic cells（DCs） and observe expression in DCs. Methods： The constructed recombinant plasmid was transfected to HEK293 cells for amplification, based on which recombinant adenovirus Ad-IL-24 was obtained and transfeeted to mouse bone marrow-derived DCs cul- tured in vitro. The expression of IL-24 in DCs were detected by RT-PCR and Western blot. The morphology of transfected DCs was observed by microscopy. Results： The recombinant adenovirus vector was successfully amplificated and transfected DCs. IL-24 were highly expressed in DCs by RT-PCR and Western blot. Conclusion： Taken together, these results indicated that recombinant Arlenevirus with IL-24 successfully infected DCs.

关 键 词：人白介素24 腺病毒 树突细胞 

分 类 号：R392.1[医药卫生—免疫学]