当归补血汤含药血清对内皮祖细胞功能及其PI3K/Akt通路影响的研究  

Influence of drug-serum containing Dangguibuxue decoction on functional activity and PI3K /Akt pathway of endothelial progenitor cells

作　　者：秦臻[1] 黄水清[2] 

机构地区：[1]贵阳医学院药理学教研室,贵州贵阳550004 [2]广州中医药大学,广东广州510405

出　　处：《中国药理学通报》2013年第9期1320-1323,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30973895)

摘　　要：目的研究当归补血汤含药血清对人内皮祖细胞(EPCs)功能及其PI3K/Akt通路的影响。方法体外培养并鉴定人外周血EPCs,制备当归补血汤含药血清,对EPCs进行不同血清或PI3K抑制剂的干预,检测EPCs的增殖、黏附、迁移、成小管功能及NO分泌量,RT-PCR检测eNOS mRNA的表达,Western blot检测Akt蛋白的表达。结果与对照组比较,当归补血汤含药血清能促进EPCs的增殖、黏附、迁移及成小管功能(P<0.05),促进EPCs分泌NO,上调细胞eNOS mRNA、总Akt和磷酸化Akt蛋白的表达,但这些作用均在PI3K抑制剂处理后明显减弱(P<0.05)。结论当归补血汤可能通过PI3K/Akt通路调节EPCs的功能。Aim To study the influence of drug-serum containing Dangguibuxue decoction（DBD） via PI3K/Akt-dependent path- way on human endothelial progenitor cells （EPCs）. Methods EPCs from human peripheral blood were cultured in vitro and i- dentified. Drug-serum containing DBD with or without PI3K in- hibitor was applied to EPCs. The proliferation, migration, adhe- sion and tubule formation of EPCs were assayed and the secretion of NO was detected. The expression of eNOS mRNA was assayed by using realtime PCR, and protein expression of Akt by Western blot analysis. Results Drug-serum containing DBD could im- prove the proliferation, migration, adhesion and tubule formationof EPCs （ P 〈 O. 05 ） , promote the cellular secretion of NO, and up-regulate the expression of eNOS mRNA,the expression of to- tal and phosphate Akt protein. However, the promoting effect on EPCs by DBD was attenuated by PI3K inhibitor. The expressions of eNOS mRNA,total and phosphate Akt protein were all down- regulated by PI3K inhibitor. Conclusion DBD could regulate the functional activities of EPCs through PI3K/Akt pathway.

关 键 词：当归补血汤 内皮祖细胞 功能 PI3K/AKT通路 一氧化氮 内皮型一氧化氮合酶 血清药理学 

分 类 号：R289.5[医药卫生—方剂学] R329.2[医药卫生—中药学]