用小鼠骨髓内皮细胞条件培养液代替造血刺激因子培养CFU-GM和HPP-CFC  

Substitution of BMEC-CM for hematopoietic stimulators in the culture of CFU-GM and HPP-CFC

作　　者：程腊梅[1] 王绮如[1] 

机构地区：[1]血液生理研究室,长沙410078

出　　处：《湖南医科大学学报》2000年第6期539-541,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金!(39970 0 92 )

摘　　要：利用大于 10kD组分的小鼠骨髓内皮细胞条件培养液 (mBMEC CM)作刺激物体外琼脂培养CFU GM ,与rmGM CSF合用培养HPP CFC获得成功。就培养CFU GM和HPP CFC而言 ,用细胞因子作刺激物与并用大于 10kD组分的mBMEC CM作刺激物相比较 ,以后者的CFU GM及HPPThe effects of >10?kD component of serum free conditioned medium from murine bone marrow derived endothelial cell line on the formation of CFU GM and HPP CFC were investigated. We found that >10?kD component alone could stimulate CFU GM colony formation in dose dependent. Above 10?kD component supplemented with GM CSF not only resulted in significant increase of CFU GM as compared with >10?kD or GM CSF alone, but also could stimulate HPP CFC formation. The effect of >10?kD component on the formation of HPP CFC was also in dose dependent. The component above 10?kD combined with IL 3, IL 6, EPO, SCF or GM CSF further enhanced HPP CFC colony formation. The results suggest that >10?kD component may be used as the substitutes of hematopoietic growth factors to stimulate the formation of CFU GM and HPP CFC. [

关 键 词：骨髓内皮细胞 CFU-GM HPP-CFC 造血细胞 

分 类 号：R392.1[医药卫生—免疫学]