伊洛马司他联合卡培他滨对人喉癌hep-2细胞的影响  

Effect of ilomastat combined with capecitabine on human laryngeal cancer hep-2 cell

作　　者：李黎[1] 张少容[1] 熊辉强[1] 高树峰[1] 兰宁[1] 

机构地区：[1]南昌大学第二附属医院耳鼻咽喉头颈外科,330006

出　　处：《重庆医学》2013年第27期3269-3271,3274,共4页Chongqing medicine

基　　金：江西省教育厅科学技术研究项目(GJJ11361)

摘　　要：目的探讨伊洛马司他联合化疗药物卡培他滨对人喉癌hep-2细胞生长的影响。方法伊洛马司他、卡培他滨两药单独和联合处理hep-2细胞,未处理组为对照组,采用甲基偶氮唑盐(MTT)法分析hep-2细胞的增殖活性,并采用金氏Q值判断联合用药的性质;逆转录-聚合酶链反应(RT-PCR)检测hep-2细胞中基质金属蛋白酶-9(MMP-9)mRNA的表达水平;流式细胞术检测hep-2细胞的凋亡率。结果伊洛马司他与卡培他滨对hep-2细胞均有抑制作用,联合用药细胞抑制率明显增高(P<0.05),两药联合浓度为(8+100)μg/mL时为协同作用,(40+400)μg/mL时为相加作用;RT-PCR示伊洛马司他组与联合用药组的MMP-9mRNA的表达水平均低于对照组(P<0.05),联合用药组MMP-9mRNA的表达水平低于伊洛马司他组(P<0.05);流式细胞术示伊洛马司他组及卡培他滨组的凋亡率高于对照组(P<0.05),联合用药组的凋亡率高于其他各组(P<0.05)。结论伊洛马司他与卡培他滨联合用药可明显增强单药对喉癌hep-2细胞的抑制效应和诱导细胞凋亡作用,伊洛马司他的作用机制可能是下调MMP-9的表达水平。Objective To explore the effect of ilomastat combined with chemotherapeutic drug capecitabine on human laryngeal cancer hep-2 cell .Methods hep-2 cells were treated by ilomastat and capecitabine alone and their combination .The untreated group was taken as the control group .The proliferation activity of the hep-2 cells was analyzed by MTT assay ,and the Jin′s Q was adopt-ed to assess the characters of combination medication of ilomastat and capecitabine ;the expression level of MMP-9mRNA in hep-2 cell was detected by RT-PCR;the apoptosis rate of hep-2 cell was detected by the flow cytometry .Results Both ilomastat and capecitabine had the inhibiting effect on the proliferation of hep-2 cell ,and the combination of ilomastat and capecitabine increased the cell inhibitory rate（P〈0 .05） ,the interaction between ilomastat and capecitabine was the synergistic effect when the combined concentration was （8＋100）μg/mL ,while the interaction between ilomastat and capecitabine was the additive action when the com-bined concentration was （40＋ 400）μg/mL ;RT-PCR analysis showed that compared with control group ,the expression level of MMP-9mRNA in the single ilomastat group and the combination group were both decreased （P〈 0 .05） ,and the expression of MMP-9mRNA in the combination group was lower than that in the single ilomastat group （P〈0 .05）;the flow cytometry indicated that the apoptosis rate of hep-2 cell in the single ilomastat group and the single capecitabine group were both higher than that in the control group（P〈0 .05） ,and the apoptosis rate in the combination group was higher than that in the other groups （P〈0 .05） .Con-clusion Ilomastat combined with capecitabine can obviously enhance the inhibition and apoptosis-induced ability of single drug on laryngeal cancer hep-2 cell ,the action mechanism of ilomastst is down-regulation of the expression level of the MMP-9 mRNA .

关 键 词：喉肿瘤 伊洛马司他 卡培他滨 

分 类 号：R96[医药卫生—药理学]