罗哌卡因对CCI模型鼠DRG神经元GABA激活膜电流的影响  

Effects of ropivacaine on GABA-activated membrane currents in isolated dorsal root ganglion neurons in CCI model rats

作　　者：杨越[1,2] 樊超[1,2] 司军强[1,3,4] 马克涛[1,3] 李丽[1,3] 

机构地区：[1]新疆地方病与民族高发病教育部重点实验室,新疆石河子832002 [2]石河子大学医学院第一附属医院麻醉科,新疆石河子832002 [3]石河子大学医学院生理教研室,新疆石河子832002 [4]武汉大学基础医学院生理教研室,430071

出　　处：《重庆医学》2013年第27期3278-3280,共3页Chongqing medicine

基　　金：兵团青年科技创新基金专项(2010JC33)

摘　　要：目的观察罗哌卡因对坐骨神经慢性压榨损伤（CCI）模型大鼠的背根神经节（DRG ）神经元γ-氨基丁酸（GABA ）激活膜电流的影响，探讨罗哌卡因可能存在的镇痛机制。方法运用全细胞膜片钳技术，分3组记录CCI模型大鼠手术侧、手术对侧及假手术组急性新鲜分离的DRG神经元在预灌流罗哌卡因30 s后，GABA受体激活电流（IGABA ）的变化。结果（1）与手术对侧、假手术组及对照组比较，CCI模型大鼠手术侧组的热缩足潜伏期显著缩短（P＜0．05）；（2）CCI手术对侧组DRG神经元上不同浓度（0．1～1000μmol/L）GABA激活电流幅值显著大于手术侧组和假手术组；（3）预灌流罗哌卡因（0．1～1000μmol/L）后CCI模型大鼠手术侧组、手术对侧及假手术组DRG神经元对100μmol/L GABA激活电流均表现为不同程度的增强作用，并且CCI手术对侧组的增强幅度显著大于手术侧组和假手术；（4）预灌流100μmol/L的罗哌卡因后CCI模型大鼠手术侧组DRG神经元GABA（0．1～1000μmol/L）激活电流的量效曲线左移，两EC50之间差异无统计学意义（P＞0．05）。结论罗哌卡因对CCI模型大鼠DRG神经元GABA激活电流具有增强作用，可能是罗哌卡因产生麻醉和镇痛作用的原因之一。Objective To investigate the effects of ropivavaine on gamma -aminobutyric acid（GABA）-activated membrane cur-rents in isolated dorsal root ganglion（DRG） neurons of the rats with ischiadic nerve chronic constriction injury （CCI） and to discuss the possible analgesia mechanism of ropivacaine .Methods The whole-cell patch clamp technique was used to record and compare the changes of GABA receptor activation currents of acute isolated DRG neurons after 30 s of ropivacaine preperfusion in the oper-ating side and the operative opposite side of the CCI model rats and the sham-operation group .Results （1）Compared with the oper-ative opposite side ,the sham-operation group and the control group ,the thermal withdrawal latency in the operative side group of the CCI model rats was notablely shortened（P〈0 .05）;（2）the amplitude of GABA-activated currents with different concentration GABA（0 .1-1 000μmol/L） in the operative opposite side group of the CCI operation was significantly greater than that of the op-erative side group and the sham-opeartion group ;（3）DRG neurons after ropivacaine preperfusion （0 .1-1 000μmol /L） showed va-rying degrees of enhancement effect on the 100 μmol/L GABA-activated currents ,the enhancement amplitude in the CCI operative opposite side group was significantly greater than that in the operative side group and the sham-operation group ;（4）The dose-re-sponse curve of DRG neurons GABA （0 .1-1 000μmol/L） activated current in the operative side group of the CCI rats after ropiva-caine pre-perfusion （100 μmol/L） was shifted to the left ,the difference between two EC50 had no statistical significance （P〉0 .05） .Conclusion Ropivacaine has the enhancement effect on GABA activated currents in the DRG neurons of the CCI model rats , which could be one of reasons for ropivacaine producing the anesthetic and analgesic effect .

关 键 词：罗哌卡因 神经病理痛 背根神经节 γ-氨基丁酸 全细胞膜片钳 

分 类 号：R614[医药卫生—麻醉学]