一种新型DNA自组装磁珠光电检测系统及其在DNA计算机研制中的应用  

A New Photoelectric DNA-Detection Platform with Assembled Magnetic Beads and Its Application on DNA Computer

作　　者：李菲[1] 许进[1] 

机构地区：[1]北京大学信息科学技术学院高可信软件技术教育部重点实验室,北京100871

出　　处：《计算机学报》2013年第9期1826-1834,共9页Chinese Journal of Computers

基　　金：国家自然科学基金(61074169;60974112;60971085;30970969);国际重大项目合作(60910002)等项目资助;863 Program of China (2009AA012413);Ph.D.Programs Foundation of Ministry of Education of China(20070001020);Postdoctoral Science Foundation of China(20080440257)~~

摘　　要：文中提出了一种基于硅芯片集成自组装磁珠颗粒的新型DNA光电检测系统,该系统利用普通照射光源及光电二极管进行光电信号转换,通过比较DNA杂交反应前后的光电流值,来识别DNA杂交信号.该系统是一种首次将磁珠和光电二极管相结合的新型DNA杂交检测系统,具有成本低廉、快速检测及高精度的特点.这种检测方法不需要信号增强步骤,就能够有效区分DNA单碱基错配及完全杂交的情况;由于采用了磁珠颗粒,易于在DNA计算中删除问题的非解.文中给出了求解图的最小顶点覆盖问题DNA计算模型实例,该实例证实了文中所提出的检测系统较传统检测系统具有明显的优势,有利于实现DNA计算机检测系统中解的自动化检测.In this work, we propose a DNA hybridization detection platform with assembled magnetic beads and integrated photodiodes on a silicon chip. Common light resource and the fabricated photodiode are utilized to turn ordinary light into electricity. Compared with the detected photocurrent before hybridization, the signal of DNA hybridization can be distinctly diagnosed by light intensity changes. The advantages of this detection platform are lower cost, higher precision and less time consuming. With this technique, we could successfully discriminate between single base mismatched DNA and perfectly matched DNA sequence, which saves the time of amplifying hybridization signal. This detection platform also fits for DNA computing because magnetic property could easily eliminate unfeasible solutions of problem. The experimental result of a minimum vertex cover problem, which is solved by DNA computing on this platform, reveals the obvious benefits of this detection platform to be used to automatically detecting solutions of DNA computing.

关 键 词：DNA检测 光电二极管 磁珠 DNA杂交反应 DNA计算 

分 类 号：TP18[自动化与计算机技术—控制理论与控制工程]