兰花炭疽病拮抗细菌5A5-3菌株的初步筛选和鉴定  

Preliminary Isolation and Identification of the Antagonistic Bacterial Strain 5A5-3 Against Common Orchid Diseases

作　　者：曹晓璐[1] 刘慧娟[2] 郭晓军[2] 姚娜[1] 李潞滨[1] 

机构地区：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,北京100091 [2]河北农业大学生命科学学院,河北保定071001

出　　处：《林业科学研究》2013年第5期598-602,共5页Forest Research

基　　金：国家林业局948项目(2011-4-71);"十二五"科技支撑计划中国特色花卉种业关键技术研究(2012BAD01B07)

摘　　要：从毛乌素沙地采集的10份土样中共分离到细菌320株,将分离到的细菌菌株与兰花炭疽病病原菌胶胞炭疽菌进行生长对峙试验。初筛获得拮抗菌57株。复筛后得到筛选自毛乌素沙地土壤生物结皮样品的5A5-3对病原菌具有较高的抑菌活性。对其进行形态特征观察和生理生化试验及16S rDNA序列相似性分析,结果表明:菌株5A5-3与Bacillus velezensis标准菌株(CR-502,AY603658)的16S rDNA序列相似度达100%,最终将菌株5A5-3鉴定为Bacillus velezensis。320 strains were isolated from 10 soil samples collected at Mu Us sandy land of China. After growing confrontation test to Colletotrichum gloeosporioide, 57 strains were screened preliminarily. The strain 5A5-3 screened from biological crusts of Mu Us sandy land with a rather higher antagonistic activity against C. gloeosporioide was ob- tained after secondary screening. The strain 5A5-3 was further identified by morphological and cultural characteris- tics, physiological and biochemical experiments and 16S rDNA sequence analysis. Based on the results, the strain was finally identified as a Bacillus velezensis. 16S rDNA sequences analysis of strain 5A5-3 revealed that it had 100% similarity to B. velezensis CR-502.

关 键 词：兰花病害 拮抗细菌 筛选 鉴定 

分 类 号：S718.8[农业科学—林学]