人脂肪源性间充质干细胞的分离培养和鉴定  

Isolation,cultivation and identification of human adipose derived mesenchymal stem cells

作　　者：赵辉[1] 吕军强[1] 贾静[1] 王松[1] 傅正[1] 车绪春[1] 陆融[1,2] 林刚 姚智[1,3] 

机构地区：[1]天津医科大学基础医学院免疫学系,天津300070 [2]深圳市康哲药业有限公司,深圳518057 [3]教育部免疫微环境与疾病重点实验室,天津300070

出　　处：《天津医科大学学报》2013年第5期358-361,436,共5页Journal of Tianjin Medical University

基　　金：高校博士点基金资助项目(20101202110001);天津市应用基础与前沿计划重点项目(09JCZDJC19700);973国家重点基础研究发展计划(2009CB918903);广东省教育部产学研结合项目(2009B090300430)

摘　　要：目的:分离培养并鉴定人脂肪源性间充质干细胞(hAD-MSC)。方法:采用贴壁法从人脂肪组织分离获得hAD-MSC,并从表面分子表达和定向分化两个方面进行鉴定。结果:成功从脂肪组织中分离获得间充质干细胞,该群细胞贴壁生长,形态类似成纤维细胞样;具有较强的体外增殖能力和自我更新能力;一致性好,纯度高,99%的细胞阳性表达CD105、CD90、CD13、CD44分子;CD117、CD34、CD45和HLA-DR阴性表达,阳性率低于2%;可向脂肪和成骨细胞定向分化。结论:分离所得细胞符合间充质干细胞基本特点,可确定为hAD-MSC。Objective： To isolate and expand human adipose derived mesenchymal stem cells （hAD-MSC） fi＇om aduh adipose tissue, and define the characterislies of these cells. Methods： hAD-MSC were isolated from adipose tissue by eollagenase digestion and adherence to plastic, and the surface marker and potential nmhipotent differentiation ability of these cells were identified. Results： Fibroblast-like cells were obtained fi＇om adipose tissue. These cells could be expanded quickly in vitro, and showed self-renewal ability. CD 105, CD90, CD 13 and CI）44 were expressed in 99% of isolated cells while CD117, CD34, CD45 and HLA-DR expression was negative. They could be in- duced to differentiate osleoblasts and adipucytes in special differentiation medium. Conchlsion：These cells from human adipose are hAD- MSC.

关 键 词：人脂肪源性间充质干细胞 分离 鉴定 定向分化 

分 类 号：Q813[生物学—生物工程]