自身免疫性甲状腺疾病中不变链的表达  

Study on the expression of invariant chain in autoimmune thyroid diseases

作　　者：王巧巧[1] 张勇[1] 杨裕国[2] 袁勤[3] 奚宏康 王福庆[1] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,200025 [2]上海第二医科大学附属第九人民医院内科 [3]上海第二医科大学附属瑞金医院检验科

出　　处：《中华内分泌代谢杂志》2000年第6期366-369,I005,I007,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金!资助项目 (395 0 0 0 71)

摘　　要：目的　探索桥本甲状腺炎与Graves病患者甲状腺滤泡上皮细胞异常表达的HLAⅡ类分子能否递呈自身抗原。方法　标记链霉亲和素结合 (LSAB)技术检测患者甲状腺滤泡上皮细胞中HLAⅡ类分子、不变链 (Ii链 )与B7分子的表达。原位杂交技术 (ISH)进一步从mRNA水平上检测Ii链的表达。结果　桥本甲状腺炎与Graves病患者甲状腺滤泡上皮中HLAⅡ类分子、Ii链与B7分子表达的格局呈高度异质性。结论　至少有一部分自身免疫性甲状腺炎患者的滤泡上皮细胞具备递呈自身抗原必要的分子基础。Objective To investigate whether the aberrantly expressed HLA class Ⅱ molecules on the follicular epithelial cells of Graves' disease and Hashimoto's thyroiditis present autoantigens. Methods Expressions of HLA class Ⅱ, invariant (Ii) chain and B7 on follicular epithelial cells were detected by labeled streptavidin binding (LSAB) and expression of Ii chain at mRNA level was assayed by in situ hybridization (ISH). Results The expression pattern of HLA class Ⅱ, Ii chain and B7 on epithelial follicular cells from Graves' disease and Hashimoto's thyroiditis is highly heterogeneous. Conclusion Follicular epithelial cells possess the necessary molecular basis for presenting autoantigens and activating T cells, at least in a part of the patients.

关 键 词：自身免疫性甲状腺炎 不变链 GRAVES病 

分 类 号：R581[医药卫生—内分泌]