红景天对低压缺氧损伤大鼠海马Parvalbumin蛋白表达的影响及机制  

THE MECHANISMS AND EFFECTS OF RHODIOLA ON EXPRESSION OF PARVALBUMIN IN RAT BRAINS AFTER HYPOBARIC HYPOXIA INJURY

作　　者：祁存芳[1,2] 黄明玉[1] 李长兴[1] 张军峰[3,2] 张建水[2] 李福鑫[1] 唐延军[1] 

机构地区：[1]青海大学医学院基础医学部解剖学教研室,青海西宁810001 [2]西安交通大学医学院教育部环境和基因相关疾病重点实验室神经生物研究所,陕西西安710061 [3]西安医学院人体解剖学教研室,陕西西安710021

出　　处：《青海医学院学报》2013年第3期167-172,共6页Journal of Qinghai Medical College

基　　金：国家自然科学基金资助项目(No.30960107);青海大学医学院中青年科技项目(2007-ky-003)

摘　　要：目的研究红景天对高原低压缺氧环境中大鼠学习记忆功能的影响,并探讨其作用机制。方法制作大鼠高原低压缺氧模型,采用主动回避反应(Active avoidance reaction,AAR)学习记忆检测和免疫组织化学染色法检测Parvalbumin(PV)蛋白表达并行相关分析研究。结果和对照组比较红景天可延缓低压缺氧环境中大鼠AAR保持率的降低,增加高原低压缺氧环境中大鼠PV阳性神经元数量和蛋白表达(P<0.05)。结论红景天能改善低压缺氧损伤对大鼠学习记忆能力的影响,其作用机制可能是通过调节PV阳性神经元数量和蛋白表达,对高原低压缺氧脑损伤发挥保护作用。Objective to observe the effects of Rhodiola on learning and memory function in rats suffered from the hypobarie hypoxia injury, and to explore the possible mechanism. Methods SD rats were randomly divided into six groups and the hypobaric hypoxia injury model was established. Then the active avoidance reaction （AAR） was performed to determine the effects of Rhodiola on learning and memory function, and the expression of Parvalbumin was detected using immunohistochemical staining. Results Compared with control group＇, the memory capacity of the rats suffered from the hypobaric hypoxia injury was improved in Rhodiola pretreatment group （ P 〈 0.05 ） , with increased expression of Parvalbumin （ P 〈 0.05 ）. Conclusion Rhodiola can protect the rat brains against the hypobaric hypoxia injuries, the mechanism which may mediated by the Parvalbumin protein expression.

关 键 词：低压缺氧 学习记忆 红景天 Parvalbumin(PV)蛋白 

分 类 号：R34[医药卫生—基础医学]