大鼠软脑膜微血管通透性的计算机分析  

IMAGE COMPUTER ANALYSIS OF MICROVASCULAR PERMEABILITY OF RAT CEREBRAL PIA MATER

作　　者：陈虹[1] 丁库克[1] 胡金麟[2] 曾衍钧[1] 

机构地区：[1]北京工业大学生物力学和医学信息研究所,北京100022 [2]中国人民解放军总医院微循环研究室,北京100853

出　　处：《中国生物医学工程学报》2000年第4期404-410,共7页Chinese Journal of Biomedical Engineering

基　　金：北京市自然科学基金

摘　　要：本文应用微循环活体观察和荧光示踪技术 ,通过计算机数字图象分析对荧光素钠 (FiNa)在软脑膜微血管的通透过程进行了定量研究 ,以互成角度的两根血管为研究对象 ,建立了正常大鼠软脑膜和缺血大鼠软脑膜微血管对荧光素钠的通透方程 ,得到了不同缺血条件下的微血管通透速度方程及对通透速度评价的定量方法。通过对所得结果进一步分析 ,计算出两血管夹角与微血管通透性之间的关系 ,并代入不同缺血条件下大鼠软脑膜的通透曲线进行了验证。结果 :大鼠软脑膜微血管的通透方程成对数分布 ,通透速度成幂函数下降 ,一段时间后趋于稳定。以缺血 +1h再灌注大鼠通透最为迅速 ,缺血 +12h再灌注大鼠通透速度略快于正常大鼠。对于互成角度的两根血管 ,荧光物质的扩散速度与两血管间夹角存在一定关系。结论 :本方法能够较好地反映软脑膜微血管通透的实际情况 ,为进一步建立复杂结构血管的通透方程 ,以及定量评价微血管物质交换参数 ,提供了数学基础。A Microcirculation intravital observation and fluorescent tracer technique were used and digital image analysis method was applied for quantitative measuring of fluorescein sodium(FiNa) permeability in cerebral pia mater microvessels. The transport permeation equations of FiNa within the blood vessels and through the vessel walls into the perivascular tissue of the normal rat and ischemic rat were established when two vessels as the objecct, thus the permeation speed equation under different ischemic conditions were deduced. Based on analysis of the results, we deduced the relation between the permeability and the included angle of two vessels. Result: logarithm model showed a good fit of the experiment data, the permeation equation showed logarithmic distribution and then tended towards stability. FiNa could pass through the wall of microvessel with the highest speed in one hour ischemia rat group, and the permeation speed of FiNa in rats received reperfusion after twelve hours of ischemia was higher faster than that in normal rat. It is expected that this method would be very useful for the quantitative analysis in cerebral pia mater microvascular permeability.

关 键 词：计算机图象分析 微血管通透性 灰度 软脑膜 

分 类 号：R331.35[医药卫生—人体生理学] TP391.41[医药卫生—基础医学]