H7N9禽流感患者外周血T淋巴细胞亚群的分析及意义  

Analysis and significance of T lymphocyte subsets in peripheral blood of patients with avian influenza A ( H7N9) virus infection

作　　者：毛会军[1] 陈戴红[1] 张敏[1] 王刚[1] 邱建萍[1] 

机构地区：[1]上海市公共卫生临床中心医学检验科,上海201508

出　　处：《检验医学》2013年第9期758-761,共4页Laboratory Medicine

摘　　要：目的探讨H7N9禽流感患者外周血T淋巴细胞亚群的变化情况。方法将18例H7N9禽流感患者(感染组)根据治疗预后分为治愈组13例和死亡组5例,以门诊健康献血者20名作为正常对照组。采用特异性引物聚合酶链反应(PCR)检测H7N9禽流感病毒,并用流式细胞术检测外周血T淋巴细胞亚群的变化。结果感染组CD3+、CD8+和CD4+T细胞绝对数及CD4+/CD8+比值均低于正常对照组(P<0.05)。治愈组CD3+、CD8+及CD4+T细胞绝对数均低于正常对照组(P<0.05)。与治疗前比较,治愈组治疗后CD3+、CD8+及CD4+T细胞绝对数明显升高(P<0.05);但CD3+、CD4+T细胞绝对数及CD4+/CD8+比值仍低于正常对照组(P<0.05)。死亡组治疗前、后CD3+、CD8+及CD4+T细胞绝对数均低于治愈组和正常对照组(P均<0.05);且治疗前、后各项指标之间差异均无统计学意义(P>0.05)。治愈组CD3+、CD8+及CD4+T细胞绝对数均高于死亡组(P<0.05)。结论 H7N9禽流感患者存在比较严重的T淋巴细胞亚群失衡,死亡患者免疫失衡更为严重。T淋巴细胞亚群失衡可能与H7N9禽流感的发病有关。Objective To investigate the changes of peripheral blood T lymphocyte subsets in avian influenza A （H7N9） virus infection patients. Methods According to the prognosis, 18 patients with avian influenza A （H7N9） virus infection （infection group） were classified into 13 cases of cure group and 5 cases of dead group. There were 20 healthy subjects as the control group. Specific primer polymerase chain reaction （PCR） was used to detect the avian influenza A （HTN9）virus, and the T lymphocyte subsets in peripheral blood were detected by flow cytometry. Results Compared with the control group, the absolute numbers of CD3 ~T cell, CD8 ＋T cell and CD4 ＋T cell and CD4 ＋/CD8 ~ ratio of the infection group decreased significantly （ P 〈 0.05 ）. In the cure group, the absolute numbers of CD3 ＋ T cell, CD8 ＋ T cell and CD4 ＋ T cell were lower than those of the control group （ P 〈 0.05 ）. In the cure group, the absolute numbers of CD3 ＋ T cell, CD8 ＋ T cell and CD4 ＋ T cell before the treatment were significantly lower than those after the treatment （ P 〈 0.05 ）, and the absolute numbers of CD3 ＋ T cell and CD4 ＋ T cell and CD4 ＋/CD8 ＋ ratio were lower than those in the control group （P 〈 0.05）. In the dead group, the absolute numbers of CD3 ＋ T cell, CD8＋ T cell and CD4＋ T cell before and after the treatment were lower than those in the cure and control groups （P 〈 0.05） , and there was no statistical significance for the all parameters before and after the treatment （P 〉 0.05 ）. Compared with the dead group, the absolute numbers of CD3 ＋ T cell, CD8 ＋ T cell and CD4＋T cell in the cure group were higher （ P 〈 0.05 ）. Conclusions There is the imbalance of T lymphocyte subsets in avian influenza A （HTN9）virus infection patients. In the dead group, the imbalance of T lymphocyte subsets is serious. The imbalance may be correlated with the pathogenesis of avian influenza A （H7N9）virus infection.

关 键 词：H7N9禽流感病毒 T淋巴细胞亚群 奥司他韦 

分 类 号：R373.1[医药卫生—病原生物学]