聚乙二醇沉淀法与凝胶色谱层析法检测巨泌乳素的相关性研究  

Study on the correlation between polyethylene glycol precipitation treatment and gel filtration chromatography in the detection of macroprolactin

作　　者：王霞[1] 刘金玲[2] 高硕[2] 

机构地区：[1]天津市血液中心,天津300110 [2]天津市医科大学总医院,天津300052

出　　处：《检验医学》2013年第9期824-827,共4页Laboratory Medicine

摘　　要：目的探讨聚乙二醇(PEG)沉淀法检测巨泌乳素(MPRL)方法的可行性。方法将26例高泌乳素血症(HPRL)患者按临床表现和影像学资料分为真性HPRL组(简称真泌组)11例及MPRL血症组(简称巨泌组)15例。采用凝胶色谱层析(GFC)法分离泌乳素(PRL)各组分,检测各组分PRL浓度,同时将所有标本进行PEG沉淀,检测处理后单体PRL浓度,并与GFC法单体PRL检测结果进行相关性分析。结果巨泌组与真泌组的PRL层析谱不同,真泌组以单体PRL为主,而巨泌组以巨PRL为主。真泌组与巨泌组血清处理前总PRL浓度差异无统计学意义(P>0.05),而处理后真泌组明显高于巨泌组(P<0.05)。PEG沉淀法与GFC法检测真泌组与巨泌组单体PRL浓度差异无统计学意义(P>0.05),且相关性良好[相关系数(r)=0.844,P<0.05]。结论PEG沉淀法检测MPRL结果与GFC法有良好的相关性,且经济、简便、易行,可作为临床常规筛查方法。Objective To investigate the feasibility of polyethylene glycol （PEG） precipitation treatment in the detection of macroprolactine（MPRL）. Methods A total of 26 hyperprolactinemia （HPRL） patients were classified into 2 groups： genuine HPRL group （ 11 cases） and macroprolactinemia group （ 15 cases） according to the clinical and imaging data. By gel filtration chromatography （ GFC）, the components of prolactin （PRL） were separated, and their levels were determined. After PEG precipitation treatment, the concentration of monomer PRL was determined, the results were compared with those by GFC, and the correlation was analyzed. Results The chromatogram of macroprolactinemia group was different from that of genuine HPRL group. The genuine HPRL group was consisted of monomer PRL, but the macroprolactinemia group was consisted of big PRL. The 2 groups＇ PRL before the treatment had no statistical significance （ P 〉 0.05 ）. After the treatment, the PRL concentrations of genuine HPRL group were high than those of macroprolactinemia group （P 〈 0. 05 ）. The 2 groups＇ monomer PRL concentrations before PEG precipitation treatment and GFC had no statistical significance （P 〉 0.05 ） , and the correlation was good [ correlation coefficient （r） = 0. 844, P 〈 0.05 ]. Conclusions PEG precipitation treatment for the detection of MRPL has good correlation with GFC, and it is economical and convenient. It is recommended as the routine screening method.

关 键 词：泌乳素 巨泌乳素 高泌乳素血症 聚乙二醇 凝胶色谱层析法 

分 类 号：R446.1[医药卫生—诊断学]