盐度对六株硅藻生长及脂肪酸组成的影响(英文)  

EFFECTS OF SALINITY ON THE GROWTH AND FATTY ACID COMPOSITION OF SIX STRAINS OF MARINE DIATOMS

作　　者：梁英[1] 麦康森[1] 孙世春[1] 黄国效 胡海燕 

机构地区：[1]青岛海洋大学教育部水产养殖重点实验室,青岛266003

出　　处：《海洋湖沼通报》2000年第4期53-62,共10页Transactions of Oceanology and Limnology

基　　金：Project supported by the Hi-Tech"863"programs China Ministry of Science and Technology(863-819-02-01)

摘　　要：在温度为 ２２±１℃，盐度为 １８‰、 ２８‰和 ３８‰的条件下，用Ｆ／２培养基对青 岛海洋大学微藻种质库保存的 ６株硅藻（纤细角毛藻 Ｃｈａｅｔｏｃｅｒｏｓｇｒａｃｉｌｉｓ１３；筒 柱藻Ｃｙｌｉｎｄｒｏｔｈｅｃａ　ｆｕｓｉｆｏｒｍｉｓＢ２１１；三角褐指藻Ｐｈａｅｏｄａｃｔｙｌｕｍ　ｔｒｉｃｏｍｕｔｕｍ　Ｂ１１４ Ｂ１１８， Ｂ２２１；新月菱形藻 Ｎｉｔｚｓｃｈｉａ　ｃｌｏｓｅｒｉｕｍ Ｂ２２２）进行培养，在指数生长期末期 进行收获，测定了６株硅藻的生长脂肪酸组成。实验结果表明：盐度对六株硅 藻的生长及脂肪酸组成均有影响，但作用结果因种而异。Ｂ１３的相对生长率随着盐 度的增加而增加；Ｂ１１４和Ｂ２２２的相对生长率随着盖度的增加而降低。Ｂ１３的干重随着盖度的增加而增加，在盐度为３８‰时达到最大值（０．２１）；Ｂ１１８、Ｂ１１４、Ｂ２２１和 Ｂ２２２的干重均在盐度为 ２８‰。时达到最大值；盐度对 Ｂ２１１的干重影响不明显（０．３５～０．３６）。 六株硅藻的主要脂肪酸为１６： ０、 １６： １（ｎ－７）和２０： ５（ｎ－３）， Ｂ２１１还含有较多的 ２０：４ｎ－６（５．６～７．４％）， Ｂ１３含有较多的 １４：０ （２０．０～３０．９％。The effect of salinity (18‰, 28‰ and 38‰) on the growth and fatty acid composition of six strains of marine diatoms Chaetoceros gracilis (B13), Cylindrotheca fusiformis (B211), Phaeodactylum tricornutm (B114, B118 and B221) and Nitzschia closterium (B222) was investigated. The relative growth rate showed an increase in B 13 and a decrease in B114 and B222 with the increase of salinity. The dry weight of B13 increased with the increase of salinity and reached its highest value (0.21) at the highest salinity (38‰). The dry weight of B114, B 1 18, B221 and B222 both reached their highest values (0.26, 0.32, 0.40 and 0.30, respectively) at salinity 28‰. No significant difference was observed in B211 (0.35-0.36). The major fatty acids of the 6 strains were 16:0, 16:l(n-7) and 20:5(n-3). B211 also had a high percentage of 20:4n-6 (5.6.4%). B13 also had a high percentage of 14:0 (20.0- 30.9%). Saturated fatty acids decreased with the increase of salinity in B21l, B221 and B222, and reached their highest values in 18‰ in B211 (41．7%), B221 (37.6%) and B222 (31.7％), and in 289‰ in B13 (48.l%), B114 (33.0%) and B118 (31 .0%). Monounsaturated fatty acids increased in B 114, B118, B221 and B222 While decreased in B13 and B211 with the increase of salinity. Polyunsaturated fatty acids had their highest Values in 18‰ in B13 (31 .3%), B114 (19.6%), B118 (23.4%) and B222 (18.6%), and in 28‰ in B211 (25.1%) and B221 (16.3%).

关 键 词：盐度 硅藻 脂肪酸 相对生长率 干重 

分 类 号：Q949.27[生物学—植物学] Q547