树舌多糖GF对肝癌细胞的增殖抑制作用及对CyclinE基因mRNA表达的影响  

Effect of Ganoderma Appanatum Polysaccharides GF on Proliferative Inhibition of Liver Cancer Cells and the Expression of CyclinE mRNA

作　　者：于水澜[1] 于英君[1] 赵阳 宋高臣[3] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]北京医药集团职工大学,北京100079 [3]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中医药学报》2013年第4期52-54,共3页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省青年科学基金(No.QC2010001);黑龙江省青年科学技术专项基金项目(No.QC06C090);黑龙江省中医药科研计划项目(No.ZHY06-Z02)

摘　　要：目的:探明树舌多糖GF(Ganoderma appanatum polysaccharides GF,GAPSGF)对SMMC7721肝癌细胞增殖及CyclinE基因mRNA表达的影响。方法:用80、40、20、10、5、2.5、1.25、0.625μg/mL树舌多糖GF分别处理肝癌细胞SMMC7721,48h后MTT法检测树舌多糖GF对SMMC7721细胞增殖的抑制情况;实时定量聚合酶链反应(Real-Time PCR)检测CyclinE mRNA表达。结果:树舌多糖GF对SMMC7721细胞具有增殖抑制作用。Real-time PCR结果显示,CyclinE mRNA的表达量明显低于模型组。结论:树舌多糖GF可下调CyclinE mRNA的表达,从而抑制SMMC7721肝癌细胞的增殖。Objective： To study the effects of Ganoderma appanatum polysaccharides GF（GAPSGF） on proliferative inhibition and CyclinE mRNA expression in hepatoma cell line SMMC7721.Methods： The effect of GAPSGF on SMMC7721 cells proliferation was studied by using MTT.Real-time PCR was used to examine the expression of mRNA of CyclinE.Results： GAPSGF could effectively inhibit proliferation of SMMC7721 cells.GAPSGF group could significantly reduce the expressions of mRNA of CyclinE in SMMC7721 cells as compared with those in control group.Conclusion： GAPSGF can inhibit cells proliferation through down-regulating CyclinE expression in SMMC7721 cells.

关 键 词：SMMC7721肝癌细胞 树舌多糖GF CYCLINE 基因 

分 类 号：R285.5[医药卫生—中药学]