干旱胁迫下‘肋果沙棘’渗透调节物及保护酶活性变化的研究  

Changes of osmoregulation substances and protective enzyme activities of ‘Hipphae neurocarpa’ under drought stress

作　　者：马玉花[1] 刘品高[1] 高英[1] 向前胜[1] 周至远[1] 

机构地区：[1]青海大学农牧学院农林系,青海西宁810016

出　　处：《甘肃农业大学学报》2013年第4期100-104,共5页Journal of Gansu Agricultural University

基　　金：中国科学院"西部之光"人才培养计划项目

摘　　要：以‘肋果沙棘’的盆栽苗为试验材料,研究了不同程度干旱胁迫处理对‘肋果沙棘’叶片渗透调节物以及保护酶活性的影响.结果表明:‘肋果沙棘’叶片中的可溶性糖含量在轻度和中度干旱胁迫下与CK相比无显著差异,而在重度干旱胁迫下其含量急剧上升;可溶性蛋白含量则呈现先升高后降低的趋势,脯氨酸和甜菜碱含量则随着干旱胁迫程度的加剧而逐渐升高;另外,干旱胁迫下‘肋果沙棘’保护酶活性的变化趋势为SOD酶和POD酶活性随着干旱胁迫程度的增强呈先升高后降低的趋势,而CAT酶活性则随着干旱胁迫加剧而增强.由此得出‘肋果沙棘’在受到干旱胁迫时通过渗透调节物和保护酶活性的调节,迅速做出生理响应,以维持细胞膜的稳定以及正常的生命活动,说明‘肋果沙棘’具有很强的抗旱保水能力.The effect of drought stress on osmoregulation substances and protective enzyme activities in leaves were studied in this paper with ‘H. neurocarpa’ cultivated in pot. The results showed that soluble sugar content had no significant difference compared with the control under mild and moderate stress, and increased significantly under severe stress. The content of soluble protein enhanced firstly, and declined lat- er. The content of proline and betaine increased gradually with the intensified of the degree of drought stress. The activity of superoxide dismutase （SOD） and peroxidase （POD） increased at first and then de- creased under drought stress;however, catalase （CAT） activity enhanced gradually with the intensified wa ter stress. It is suggested that ‘H. neurocarpa＇ could response rapidly to maintain cell membrane stability and to keep formal physiological action through osmotic and protective enzyme regulation when suffered from drought stress. The results indicated that ‘H. neurocarp＇a have a higher capacity of drought-resistance.

关 键 词：肋果沙棘 干旱胁迫 渗透调节物 保护酶活性 

分 类 号：S722.5[农业科学—林木遗传育种]